UAS regulatory sequences drive expression of InR in which most of the alpha subunit has been deleted. This mimics a constitutively active human InR described by Ellis et al., 1987 (PMID:2842659).
decreased cell number | adult stage | somatic clone, with Scer\GAL4Tub.PU
visible, with Scer\GAL4Bx-MS1096
visible, with Scer\GAL4GMR.PF
adult midgut | somatic clone, with Scer\GAL4Tub.PU
eye, with Scer\GAL4GMR.PF
wing, with Scer\GAL4Bx-MS1096
Expression of InRdel.UAS under the control of Scer\GAL4Tub.PU in MARCM clones leads to a significant decrease in midgut cell number when compared to control adults.
Ectopic expression of InRdel.Scer\UAS in the larval body, under the control of Scer\GAL4c564, results in a 2.4-fold increase in cell size, compared to controls.
Expression of InRdel.Scer\UAS under the control of Scer\GAL4Bx-MS1096 results in a down-curled wing phenotype in 95.5% of flies.
Expression of InRdel.Scer\UAS under the control of Scer\GAL4GMR.PF results in an eye overgrowth phenotype.
InRdel.UAS, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF has visible phenotype, suppressible by Lnk6S2/Lnk4Q3
InRdel.UAS, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF has eye phenotype, suppressible by Lnk6S2/Lnk4Q3
The eye overgrowth phenotype caused by expression of InRdel.Scer\UAS under the control of Scer\GAL4GMR.PF is suppressed by Lnk4Q3/Lnk6S2.