FlyBase Allele Report: Dmel\Mcr[1]

General Information

Symbol

Dmel\Mcr¹

Species

D. melanogaster

Name

FlyBase ID

FBal0156624

Feature type

allele

Associated gene

Dmel\Mcr

Associated Insertion(s)

Carried in Construct

Also Known As

E(br)155

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Ward et al., 2003)

Progenitor genotype

Cytology

Description

Amino acid replacement: S282L.

(Hall et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:8,079,766..8,079,766 [-]

Nucleotide change:

C8079766T

Amino acid change:

S282L | Mcr-PA

Reported amino acid change:

S282L

Comment:

Reported to be at coordinate 2L:8079766 of GB:AE014134.5.

(Hall et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage

(Hall et al., 2014)

lethal - all die during embryonic stage | recessive

(Ward et al., 2003)

some die during embryonic stage | recessive

(Ward et al., 2003)

visible | semidominant

(Ward et al., 2003)

Phenotype Manifest In

embryonic/first instar larval cuticle | anterior

(Ward et al., 2003)

embryonic/first instar larval cuticle | dorsal

(Ward et al., 2003)

embryonic/larval hindgut

(Hall et al., 2014)

embryonic/larval salivary gland

(Hall et al., 2014)

larval tracheal system

(Hall et al., 2014)

leg

(Ward et al., 2003)

septate junction

(Hall et al., 2014)

ventral denticle belt

(Hall et al., 2014)

Detailed Description

Statement

Reference

Mutant embryos lack nearly all ventral denticle belts, have convoluted trachea and have ectopic deposits in the region of the salivary glands. Defects in dorsal closure are sometimes seen.

Mutant embryos show defects in septate junction structure in the salivary glands, hindgut and trachea (assayed by cora protein localisation). The dorsal tracheal trunk epithelium allows dye to pass between the haemocoel and the tracheal lumen in a dye exclusion assay in mutant stage 17 embryos, indicating a failure in paracellular barrier function.

(Hall et al., 2014)

7% of heterozygotes show a malformed leg phenotype. Homozygotes show 97 +/- 1% embryonic lethality and nearly 50% fail to complete dorsal closure.

(Ward et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

E(br)155[+]/Mcr¹ is an enhancer of visible phenotype of br¹

(Ward et al., 2003)

Other

Statement

Reference

Mcr¹, Rho1^J3.8/Rho1[+] has visible | dominant phenotype

(Ward et al., 2003)

E(br)155[+]/Mcr¹, Rho1^J3.8 has visible phenotype

(Ward et al., 2003)

Phenotype Manifest In

Enhancer of

Statement

Reference

E(br)155[+]/Mcr¹ is an enhancer of leg phenotype of br¹

(Ward et al., 2003)

Other

Statement

Reference

Mcr¹, Rho1^J3.8/Rho1[+] has leg phenotype

(Ward et al., 2003)

Mcr¹, Rho1^J3.8 has leg phenotype

(Ward et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

29% of br¹ ; E(br)155^Ebr155/+ flies show a malformed leg phenotype. The predominant leg phenotype in br¹/Y ; E(br)155^Ebr155/+ flies is short, fat tarsal segments with normal femurs and tibias. 7% of br⁵/+ ; E(br)155^Ebr155/+ flies show a malformed leg phenotype. 57% of E(br)155^Ebr155 Rho1^J3.8 double heterozygotes show a malformed leg phenotype in at least one leg. 0% of E(br)155^Ebr155 Rho1^k02107b double heterozygotes show a malformed leg phenotype in at least one leg. 16% of E(br)155^Ebr155 Rho1^E3.10 double heterozygotes show a malformed leg phenotype in at least one leg. 6% of E(br)155^Ebr155 RhoGEF2⁰⁴²⁹¹ double heterozygotes show a malformed leg phenotype in at least one leg. 1% of E(br)155^Ebr155 RhoGEF2^11-3 double heterozygotes show a malformed leg phenotype in at least one leg. 7% of E(br)155^Ebr155 zip^Ebr double heterozygotes show a malformed leg phenotype in at least one leg. 1% of E(br)155^Ebr155 zip¹ double heterozygotes show a malformed leg phenotype in at least one leg. 4% of E(br)155^Ebr155 zip^33-1 double heterozygotes show a malformed leg phenotype in at least one leg.

(Ward et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Separable from: uif¹.

(Hall et al., 2014)

Selected as: a mutation that enhances the br¹ malformed leg phenotype.

(Ward et al., 2003)

The uif¹ allele is a second site mutation on the E(br)155^Ebr155 chromosome. It has not been possible to recombine the two mutations aprt.

(Zhang and Ward, 2009)

The Mcr¹ and uif¹ mutations were originally recovered on the same "E(br)155" chromosome. The two mutations have been recombined apart.

(Hall et al., 2014)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

E(br)155

(Hall et al., 2014, Zhang and Ward, 2009, Ward et al., 2003)

E(br)155^Ebr155

Mcr¹

(Hall et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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