FlyBase Allele Report: Dmel\pAbp[EY11561]

General Information

Symbol

Dmel\pAbp^EY11561

Species

D. melanogaster

Name

FlyBase ID

FBal0158843

Feature type

allele

Associated gene

Dmel\pAbp

Associated Insertion(s)

P{EPgy2}pAbp^EY11561

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EPgy2}pAbp^EY11561

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EPgy2}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:18,140,745..18,140,745 [+]

(Pertceva et al., 2010, Cziko et al., 2009, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis (with pAbp^k10109)

(Pertceva et al., 2010)

abnormal meiotic cell cycle (with Df(2R)Pcl7B)

(Blagden et al., 2009)

female fertile (with Df(2R)Pcl7B)

(Blagden et al., 2009)

male sterile (with Df(2R)Pcl7B)

(Blagden et al., 2009)

viable (with Df(2R)Pcl7B)

(Blagden et al., 2009)

Phenotype Manifest In

centriole & spermatocyte (with Df(2R)Pcl7B)

(Blagden et al., 2009)

elongation stage spermatid (with pAbp^k10109)

(Pertceva et al., 2010)

larval multidendritic class IV neuron | somatic clone, with Scer\GAL4^Act.PU

(Cziko et al., 2009)

mitochondrion (with pAbp^k10109)

(Pertceva et al., 2010)

Nebenkern (with pAbp^k10109)

(Pertceva et al., 2010)

nucleus (with pAbp^k10109)

(Pertceva et al., 2010)

onion stage spermatid (with pAbp^k10109)

(Pertceva et al., 2010)

spindle & spermatocyte (with Df(2R)Pcl7B)

(Blagden et al., 2009)

Detailed Description

Statement

Reference

pAbp^k10109/pAbp^EY11561 heterozygotes exhibit abnormalities in spermatogenesis. these include absence of meiotic cytokinesis (in 5% of cases), resulting in formation of polyploid spermatids and defects in spermatid elongation (in approximately 100% of cases). Nuclei fail to elongate, keep a round shape and are dispersed along the cyst instead of grouping at the head of the cyst. Nebenkern DAPi staining (for DNA) appears to be non-uniform in these mutants, reflecting the effect of the trans-heterozygous mutants on mitochondrial DNA organization in at least 60% of cases. There appears to be no difference between the trans-heterozygous mutant and controls, showing that mutant mitochondrial membranes keep their integrity, but in the mutant, the onion-stage Nebenkern looks larger than in controls, and its morphology differs from a perfect sphere. Examination with an electron microscope reveals abnormal mitochondrial membranes at onion-stage spermatids in the trans-heterozygous mutant. These mitochondria also exhibit defective attachment to axonemes in the elongating spermatids.

(Pertceva et al., 2010)

pAbp^EY11561/Df(2R)Pcl7B spermatocytes show defects in meiosis; some spermatocytes have short spindles, and premature separation of centrioles is seen in some cases.

(Blagden et al., 2009)

Over-expression of pAbp^EY11561 reduces dendrite branching, and alters dendritic morphology in larval class IV sensory neurons (induced under the control of Scer\GAL4^Act.PU). Total dendrite length is not significantly reduced in these neurons.

(Cziko et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

pAbp^EY11561/pAbp^EY11561 is an enhancer of neoplasia | adult stage | male phenotype of tut⁴

(Shan et al., 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

pAbp^EY11561/pAbp^EY11561 is an enhancer of testis phenotype of tut⁴

(Shan et al., 2017)

pAbp^EY11561/pAbp^EY11561 is an enhancer of spermatogonium phenotype of tut⁴

(Shan et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The severity of the tut⁴ mutant tumorigenic phenotype is also strongly enhanced by combination with pAbp^EY11561 homozygosity, but if combined with just one copy of tut⁴, this genetic perturbation does not cause a significant tumor phenotype.

(Shan et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

20684

y¹ w^67c23; P{EPgy2}pAbp^EY11561

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

pAbp^EY11561

(Shan et al., 2017, Blagden et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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