FlyBase Allele Report: Dmel\Orai[EY09167]

General Information

Symbol

Dmel\Orai^EY09167

Species

D. melanogaster

Name

FlyBase ID

FBal0158867

Feature type

allele

Associated gene

Dmel\Orai

Associated Insertion(s)

P{EPgy2}Orai^EY09167

Carried in Construct

Also Known As

Orai³

Key Links

Genomic Maps

JBrowse

Allele class

hypermorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypermorphic allele - molecular evidence

(Pathak et al., 2015)

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EPgy2}Orai^EY09167

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EPgy2}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:17,855,150..17,855,150 [-]

(Pathak et al., 2015, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

wing

(Pathak et al., 2015)

Detailed Description

Statement

Reference

olf186-F^EY09167 homozygous larvae are smaller and developmentally delayed and majority of them die as third instar larvae, the body size of the surviving adults is significantly reduced compared to controls.

(Pathak et al., 2017)

Adult viability of olf186-F^EY09167 mutants is found to be approximately 20%. Their lethality is very likely in the larval stages because all pupae eclosed as adults.

Adults that enclose appear smaller than wild-type and have outspread wings. olf186-F^EY09167 mutants are flightless.

The normal pattern of action potentials in response to an air puff is absent in olf186-F^EY09167 flies.

(Pathak et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

olf186-F[+]/Orai^EY09167, Stim^ko has partially lethal - majority live phenotype

(Pathak et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Stim^ko/+;olf186-F^EY09167/+ double heterozygotes show reduced eclosion rate as significantly fewer adults emerge compared to wild-type and the single heterozygotes.

(Pathak et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Orai^EY09167 is rescued by Scer\GAL4^elav-C155/Orai^UAS.cZa

(Pathak et al., 2017)

Partially rescued by

Orai^EY09167 is partially rescued by Scer\GAL4^ple.PF/Orai^UAS.cZa

(Pathak et al., 2017)

Orai^EY09167 is partially rescued by Scer\GAL4^VGlut1-OK371/Orai^UAS.cZa

(Pathak et al., 2017)

Orai^EY09167 is partially rescued by Orai^UAS.cPa/Scer\GAL4^GMR57C10

(Pathak et al., 2015)

Orai^EY09167 is partially rescued by Orai^UAS.cPa/Scer\GAL4^Ddc.PU

(Pathak et al., 2015)

Orai^EY09167 is partially rescued by Scer\GAL4^ple.PF/Orai^UAS.cPa

(Pathak et al., 2015)

Not rescued by

Orai^EY09167 is not rescued by Scer\GAL4^dimm-929/Orai^UAS.cZa

(Pathak et al., 2017)

Orai^EY09167 is not rescued by Scer\GAL4^Mef2.PR/Orai^UAS.cZa

(Pathak et al., 2017)

Orai^EY09167 is not rescued by Orai^UAS.cPa/Scer\GAL4^Mef2.PR

(Pathak et al., 2015)

Orai^EY09167 is not rescued by Orai^UAS.cPa/Scer\GAL4^VGlut1-OK371

(Pathak et al., 2015)

Comments

The developmental delay, small body size and partial lethality of olf186-F^EY09167 homozygous mutants is rescued by expression of olf186-F^Scer\UAS.cZa under the control of the Scer\GAL4^elav-C155 driver. The lethality is also partially rescued when the expression is driven by either Scer\GAL4^ple.PF or Scer\GAL4^VGlut-OK371, no rescue is observed with either Scer\GAL4^dimm-929 or Scer\GAL4^Mef2.PR drivers.

(Pathak et al., 2017)

A small but significant rescue of the flight deficit in olf186-F^EY09167 is obtained by neuronal expression of olf186-F^Scer\UAS.cPa under the control of Scer\GAL4^GMR57C10.

(Pathak et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

17538

y¹ w^67c23; P{EPgy2}Orai^EY09167/In(2LR)Gla, wg^Gla-1

Kyoto

114476

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{neoFRT}42D P{EPgy2}Orai^EY09167 /CyO y⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Orai³

(Richhariya et al., 2017)

Orai^EY09167

dOrai³

(Deb et al., 2016)

dOrai^EY09167

(Balasubramanian and Srinivasan, 2020)

olf186-F^EY09167

(Pathak et al., 2015)

orai³

(Pathak et al., 2017, Pathak et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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