FlyBase Allele Report: Dmel\eca[1]

General Information

Symbol

Dmel\eca¹

Species

D. melanogaster

Name

FlyBase ID

FBal0159276

Feature type

allele

Associated gene

Dmel\eca

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: W201term.

(Bartoszewski et al., 2004)

Nucleotide substitution: G?A.

(Bartoszewski et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:9,684,715..9,684,715 [-]

Nucleotide change:

G9684715A

Reported nucleotide change:

G?A

Amino acid change:

W201term | eca-PA

Reported amino acid change:

W201term

(Bartoszewski et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile (with Df(3R)GB104)

(Saleem et al., 2012)

female sterile | recessive

(Bartoszewski et al., 2004)

lethal | maternal effect

(Bartoszewski et al., 2004)

male semi-sterile | recessive

(Bartoszewski et al., 2004)

Phenotype Manifest In

embryonic/first instar larval cuticle | dorsal | germline clone

(Bartoszewski et al., 2004)

wing vein | ectopic

(Bartoszewski et al., 2004)

Detailed Description

Statement

Reference

eca¹/Df(3R)GB104 mutant females that have been mated with wild type males are unable to lay eggs. However they are able to ovulate and move eggs through the uterus, as 55% of flies have an egg lodged in their uterus and the remainder have an egg in the oviduct. eca¹/Df(3R)GB104 heterozygotes are able to lay eggs and only 8% of females have an egg in the uterus upon dissection. These oviposition defects are not rescued when the flies are fed either octopamine or glutamate.

(Saleem et al., 2012)

Embryos derived from homozygous eca¹ germ-line clones exhibit a weak ventralised phenotype. Homozygotes show reduced survival, eclosing at a rate between 0.1 to 20% of the expected number. Homozygous or hemizygous males show reduced fertility and females lay no eggs, although many developed late oocytes are present in their ovaries which appear morphologically normal. eca¹/Df(3R)GB104 animals raised at 19^oC exhibit some thickening of the wing veins.

(Bartoszewski et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

eca¹ has embryonic/first instar larval cuticle | dorsal | germline clone phenotype, non-enhanceable by bai¹

(Bartoszewski et al., 2004)

NOT suppressed by

Statement

Reference

eca¹ has embryonic/first instar larval cuticle | dorsal | germline clone phenotype, non-suppressible by bai¹

(Bartoszewski et al., 2004)

NOT Enhancer of

Statement

Reference

eca¹ is a non-enhancer of embryonic/first instar larval cuticle | dorsal | germline clone phenotype of bai¹

(Bartoszewski et al., 2004)

Suppressor of

Statement

Reference

eca¹ is a suppressor of embryonic/first instar larval cuticle | ventral phenotype of dl¹⁵

(Bartoszewski et al., 2004)

NOT Suppressor of

Statement

Reference

eca¹ is a non-suppressor of embryonic/first instar larval cuticle | dorsal | germline clone phenotype of bai¹

(Bartoszewski et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos lacking maternal eca (eca¹) and also lacking zygotic tkv (tkv^SL144) are completely ventralised. Homozygous dl¹⁵, eca¹ germ-line clones produce embryos that form cuticles lateralised at the filzkorper level - a long dorsalised cuticle with a characteristic filzkorper ring. Embryos lacking maternal eca (eca¹) and also lacking zygotic tkv (tkv^SL144) are completely ventralised.

(Bartoszewski et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

eca¹ is rescued by Scer\GAL4^CY2/Scer\GAL4^{VP16.nanos.UTR}/eca^UAS.cBa

(Bartoszewski et al., 2004)

Partially rescued by

Df(3R)GB104/eca¹ is partially rescued by Scer\GAL4^CY2/eca^UASp.cSa

(Saleem et al., 2012)

eca¹ is partially rescued by Scer\GAL4^CY2/eca^UAS.cBa

(Bartoszewski et al., 2004)

Not rescued by

Df(3R)GB104/eca¹ is not rescued by eca^UASp.cSa/Scer\GAL4^C164

(Saleem et al., 2012)

Comments

Expression of eca^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^CY2 partially rescues the egg deposition defects seen in eca¹/Df(3R)GB104. Females are able to lay an average of 8 eggs per day, compared to zero in eca¹/Df(3R)GB104 mutants.

Expression of eca^{Scer\UAS.P\T.cSa} in octopaminergic neurons under the control of Scer\GAL4^C164 fails to rescue the egg deposition defects seen in eca¹/Df(3R)GB104 mutant females.

(Saleem et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

eca¹

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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