FlyBase Allele Report: Dmel\Jra[RNAi.UAS]

General Information

Symbol

Dmel\Jra^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0160270

Feature type

allele

Associated gene

Dmel\Jra

Associated Insertion(s)

Carried in Construct

P{UAS-Jra.RNAi}

Also Known As

UAS-jun^RNAi

Key Links

Transgenic product class

RNAi_reagent

(Jindra et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Jindra et al., 2004)

Mutagen

in vitro construct

(Jindra et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-Jra.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of a hairpin loop of Jra sequences.

(Jindra et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Jra

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^pnr-MD237

(Külshammer et al., 2015, Jindra et al., 2004)

Phenotype Manifest In

adult thorax, with Scer\GAL4^pnr-MD237

(Jindra et al., 2004)

thorax, with Scer\GAL4^pnr-MD237

(Külshammer et al., 2015)

Detailed Description

Statement

Reference

Expression of Jra^{dsRNA.Scer\UAS} under the control of Scer\GAL4^pnr-MD237 results in a cleft thorax phenotype.

(Külshammer et al., 2015)

23% of flies expressing Jra^{dsRNA.Scer\UAS} under the control of Scer\GAL4^pnr-MD237 have mild to severe defects in thorax fusion.

(Jindra et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Jra^RNAi.UAS, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Atf3^UAS.cSa, Scer\GAL4^GMR.PF

(Sekyrova et al., 2010)

Jra^RNAi.UAS, Scer\GAL4^ppl.PP is a suppressor of lethal | pharate adult stage phenotype of Atf3^UAS.cSa, Scer\GAL4^ppl.PP

(Sekyrova et al., 2010)

Jra^RNAi.UAS, Scer\GAL4^ppl.PP is a suppressor of visible phenotype of Atf3^UAS.cSa, Scer\GAL4^ppl.PP

(Sekyrova et al., 2010)

NOT Suppressor of

Statement

Reference

Jra^RNAi.UAS, Scer\GAL4^Act.PU is a non-suppressor | somatic clone of majority die during third instar larval stage | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib¹

(Külshammer et al., 2015)

Jra^RNAi.UAS, Scer\GAL4^Act.PU is a non-suppressor | somatic clone of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib¹

(Külshammer et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Jra^RNAi.UAS, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Atf3^UAS.cSa, Scer\GAL4^GMR.PF

(Sekyrova et al., 2010)

Jra^RNAi.UAS, Scer\GAL4^ppl.PP is a suppressor of adult abdomen | dorsal phenotype of Atf3^UAS.cSa, Scer\GAL4^ppl.PP

(Sekyrova et al., 2010)

Jra^RNAi.UAS, Scer\GAL4^ppl.PP is a suppressor of adult cuticle | dorsal phenotype of Atf3^UAS.cSa, Scer\GAL4^ppl.PP

(Sekyrova et al., 2010)

NOT Suppressor of

Statement

Reference

Jra^RNAi.UAS, Scer\GAL4^Act.PU is a non-suppressor | somatic clone of eye-antennal disc | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib¹

(Külshammer et al., 2015)

Jra^RNAi.UAS, Scer\GAL4^Act.PU is a non-suppressor | somatic clone of photoreceptor neuron | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib¹

(Külshammer et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Clonal co-expression of Jra^{dsRNA.Scer\UAS} fails to suppress the lethality and developmental delay seen in larvae with scrib¹ mutant eye-antennal disc clones expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act.PU, and fails to suppress the invasive infiltration of the ventral nerve cord exhibited by cells of these clones.

Clonal co-expression of Jra^{dsRNA.Scer\UAS} fails to suppress the defective photoreceptor differentiation seen in scrib¹ mutant eye-antennal disc clones expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act.PU.

(Külshammer et al., 2015)

The mutant eye phenotype caused by expression of Atf3^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF is completely suppressed by co-expression of Jra^{dsRNA.Scer\UAS}.

Co-expression of Jra^{dsRNA.Scer\UAS} restores viability in animals expressing Atf3^Scer\UAS.cSa under the control of Scer\GAL4^ppl.PP and 87% of the rescued adults eclose with a completely normal abdomen.

(Sekyrova et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Yanicostas

(Jindra et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Jra^RNAi.UAS

Jra^{dsRNA.Scer\UAS}

Jra^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

Open Close