FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Eaat1RNAi.UAS
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General Information
Symbol
Dmel\Eaat1RNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0160571
Feature type
allele
Associated gene
Dmel\Eaat1
Associated Insertion(s)
Carried in Construct
P{UAS-Eaat1.RNAi}
Also Known As
UAS-dEAAT1-IR
Key Links
Transgenic product class
RNAi_reagent
(Rival et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Rival et al., 2004)
Mutagen
in vitro construct
(Rival et al., 2004, Rival et al., 2004)
Progenitor genotype
Carried in construct
P{UAS-Eaat1.RNAi}
Cytology
Description

UAS sequences drive expression of an inverted repeat of a 954bp fragment of the Eaat1 gene.

(Rival et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Eaat1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  neurodegenerative disease
      CEA
      (Rival et al., 2004)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  tauopathy
      modeled by Hsap\MAPTlexAop.2N4R
      (Kilian et al., 2017)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Dorsal longitudinal muscle synapses of flies expressing Eaat1dsRNA.Scer\UAS under the control of Scer\GAL4Eaat1.PR exhibit enhanced short-term depression during train simulations at higher stimulation frequencies compared with wild-type controls.

      (Danjo et al., 2011)

      Expression of Eaat1dsRNA.Scer\UAS under the control of Scer\GAL4Eaat1.PR results in viable adult flies exhibiting striking motor phenotypes. Although the mutant flies can walk and climb normally, they are reluctant to to start flying and unable to sustain flight. The mutant flies can not easily right themselves when lying down on their back. The motor axons and their associated glial processes appear morphologically normal in flies expressing Eaat1dsRNA.Scer\UAS under the control of Scer\GAL4Eaat1.PR.

      Adult neuromuscular junctions of the mutant flies expressing Eaat1dsRNA.Scer\UAS under the control of Scer\GAL4Eaat1.PR are functional. No significant difference is observed between the amplitudes of the single stimulus-evoked excitatory postsynaptic potentials (EPSPs). However, the time courses of EPSPs from the mutant flies are different from that of wild-type flies, with significantly increased half-peak widths. The neuromuscular junctions of the mutant flies display extended duration of individual EPSPs under conditions of repeated stimulation. Another striking electrophysiological abnormality that characterises the Eaat1dsRNA.Scer\UAS-expressing flies is the abnormal incidence of bursts of presynaptic spontaneous discharges which is never observed in wild-type control preparations.

      (Rival et al., 2006)

      When Eaat1dsRNA.Scer\UAS is driven by Scer\GAL4Eaat1.PR, the mutant flies exhibit striking neurological phenotypes. They can walk normally, but fly poorly, and do not try to escape when touched. These flies are generally hypoactive, but over-react when startled. These phenotypes do not appear to be due to neuromuscular dysfunction - neuromuscular junctions remain active. Mutant flies also have a significant reduction in life span. They only survive 10 to 13 days at 29oC. Mutant flies also exhibit degeneration throughout the neuropil. Mitochondria are often swollen and distended. Dopaminergic neurons appear to be particularly affected.

      (Rival et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of Hsap\EAA2Scer\UAS.cRa with Eaat1dsRNA.Scer\UAS under the control of Scer\GAL4Eaat1.PR results in the reversal of the electrophysiological phenotype resulting from Eaat1dsRNA.Scer\UAS-expression.

      (Rival et al., 2006)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Eaat1RNAi.UAS
      Eaat1dsRNA.Scer\UAS
      Eaat1dsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (7)