FlyBase Allele Report: Dmel\Smu1[f03090]

General Information

Symbol

Dmel\Smu1^f03090

Species

D. melanogaster

Name

FlyBase ID

FBal0161280

Feature type

allele

Associated gene

Dmel\Smu1

Associated Insertion(s)

PBac{WH}Smu1^f03090

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

piggyBac activity

Nature of the Allele

Allele class

Mutagen

piggyBac activity

(Bloomington Drosophila Stock Center, 2004.11.6)

Progenitor genotype

Associated Insertion(s)

PBac{WH}Smu1^f03090

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{WH}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:18,980,758..18,980,959 [+]

(Beck et al., 2012, Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage (with Df(3R)Exel6182)

(Beck et al., 2012)

abnormal neurophysiology | third instar larval stage (with Df(3R)Exel6182)

(Beck et al., 2012)

Phenotype Manifest In

NMJ bouton | third instar larval stage (with Df(3R)Exel6182)

(Beck et al., 2012)

Detailed Description

Statement

Reference

Smu1^f03090/Df(3R)Exel6182 larvae have decreased numbers of NMJ synaptic boutons compared to wild type, but a significant increase in the area of boutons present, with the result that the total presynaptic area is increased compared to wild type.

Smu1^f03090/Df(3R)Exel6182 animals have a 43% decrease in EJP amplitude at the larval NMJ compared with wild type.

(Beck et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Smu1^f03090/Df(3R)Exel6182 has abnormal neuroanatomy | third instar larval stage phenotype, non-enhanceable by Df(3R)Exel6151/beag^EP3260

(Beck et al., 2012)

Suppressed by

Statement

Reference

Smu1^f03090/Df(3R)Exel6182 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/beag^UAS.cBa

(Beck et al., 2012)

Smu1^f03090/Df(3R)Exel6182 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Fas2^UAS.cLb/Scer\GAL4^RapGAP1-OK6

(Beck et al., 2012)

NOT Enhancer of

Statement

Reference

Smu1^f03090/Df(3R)Exel6182 is a non-enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Df(3R)Exel6151/beag^EP3260

(Beck et al., 2012)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Smu1^f03090/Df(3R)Exel6182 has NMJ bouton | third instar larval stage phenotype, non-enhanceable by Df(3R)Exel6151/beag^EP3260

(Beck et al., 2012)

Suppressed by

Statement

Reference

Smu1^f03090/Df(3R)Exel6182 has NMJ bouton | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/beag^UAS.cBa

(Beck et al., 2012)

Smu1^f03090/Df(3R)Exel6182 has NMJ bouton | third instar larval stage phenotype, suppressible | partially by Fas2^UAS.cLb/Scer\GAL4^RapGAP1-OK6

(Beck et al., 2012)

NOT Enhancer of

Statement

Reference

Smu1^f03090/Df(3R)Exel6182 is a non-enhancer of NMJ bouton | third instar larval stage phenotype of Df(3R)Exel6151/beag^EP3260

(Beck et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The decrease in synaptic bouton number in beag^EP3260/Df(3R)Exel6151, Smu1^f03090/Df(3R)Exel6182 double mutants is not significantly different from that observed in either single hemizygous mutant.

Scer\GAL4^elav-C155-mediated overexpression of beag^Scer\UAS.cBa results in a 28% increase in bouton number of Smu1^f03090/Df(3R)Exel6182 mutants.

Scer\GAL4^Rapgap1-OK6-mediated expression of Fas2^Scer\UAS.cLb partially rescues synaptic bouton number Smu1^f03090/Df(3R)Exel6182 animals.

(Beck et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Smu1^f03090/Df(3R)Exel6182 is rescued by Smu1^UAS.cBa/Scer\GAL4^RapGAP1-OK6

(Beck et al., 2012)

Smu1^f03090/Df(3R)Exel6182 is rescued by Scer\GAL4^elav-C155/Smu1^UAS.cBa

(Beck et al., 2012)