FlyBase Allele Report: Dmel\escl[d01514]

General Information

Symbol

Dmel\escl^d01514

Species

D. melanogaster

Name

FlyBase ID

FBal0162083

Feature type

allele

Associated gene

Dmel\escl

Associated Insertion(s)

P{XP}escl^d01514

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Bloomington Drosophila Stock Center, 2004.11.6)

Progenitor genotype

Associated Insertion(s)

P{XP}escl^d01514

Cytology

Description

Insertion in the coding region, 8bp downstream of the ATG start codon. There is a 7bp duplication at the site of the insertion.

(Ohno et al., 2008)

Allele components

Component

Use(s)

Inserted element

P{XP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:11,990,983..11,990,983 [-]

(Ohno et al., 2008, Gene Disruption Project members and Exelixis, 2005, Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Ohno et al., 2008, Bloomington Drosophila Stock Center, 2004.11.6)

viable

(Ohno et al., 2008, Bloomington Drosophila Stock Center, 2004.11.6)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

esc⁶, escl^d01514 has lethal | P-stage phenotype

(Margueron et al., 2009)

esc⁶, escl^d01514/escl[+] has visible | recessive | homeotic phenotype

(Ohno et al., 2008)

esc⁶, escl^d01514 has lethal | recessive | pupal stage phenotype

(Ohno et al., 2008)

esc⁶, escl^d01514 has abnormal developmental rate | third instar larval stage phenotype

(Ohno et al., 2008)

Phenotype Manifest In

Other

Statement

Reference

esc⁶, escl^d01514 has imaginal disc phenotype

(Margueron et al., 2009)

esc⁶, escl^d01514 has brain phenotype

(Margueron et al., 2009)

esc⁶, escl^d01514 has eye disc phenotype

(Ohno et al., 2008)

esc⁶, escl^d01514 has wing disc phenotype

(Ohno et al., 2008)

esc⁶, escl^d01514/escl[+] has adult abdominal segment 4 phenotype

(Ohno et al., 2008)

esc⁶, escl^d01514/escl[+] has adult abdominal segment 5 | ectopic phenotype

(Ohno et al., 2008)

esc⁶, escl^d01514/escl[+] has haltere | ectopic phenotype

(Ohno et al., 2008)

esc⁶, escl^d01514 has embryonic/larval optic lobe phenotype

(Ohno et al., 2008)

esc⁶, escl^d01514 has haltere disc phenotype

(Ohno et al., 2008)

esc⁶, escl^d01514/escl[+] has sex comb | ectopic phenotype

(Ohno et al., 2008)

esc⁶, escl^d01514/escl[+] has wing phenotype

(Ohno et al., 2008)

esc⁶, escl^d01514/escl[+] has leg | ectopic phenotype

(Ohno et al., 2008)

esc⁶, escl^d01514/escl[+] has antenna phenotype

(Ohno et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Flies zygotically mutant for both esc⁶ and escl^d01514 produce larvae with poorly developed brain and imaginal discs, which die when they pupate.

The lethality of esc⁶, escl^d01514 double mutants is completely rescued by esc^T:Hsap\MYC.

The lethality of esc⁶, escl^d01514 double mutants is not rescued by esc^{F77A.T:Hsap\MYC}, although the brain and imaginal disc phenotypes are alleviated by esc^{F77A.T:Hsap\MYC}.

The lethality of esc⁶, escl^d01514 double mutants is not rescued by esc^{F345A.T:Hsap\MYC}.

(Margueron et al., 2009)

Homozygous esc⁶ escl^d01514 double mutants which have received maternal esc[+] function (as the female parents carry an esc[+] transgene on a balancer chromosome) survive through a prolonged third larval instar and die as early pupae. The double mutant larvae grow slowly. They have very small haltere and wing imaginal discs and the eye part of the eye-antenna disc is reduced in size, while the leg discs are nearly normal in size. The abdominal ganglia of the central nervous system are nearly normal in size in the double mutant larvae, but the optic lobes are approximately half normal diameter.

Adult flies that are homozygous for escl^d01514 but heterozygous for esc⁶ are viable, fertile and appear normal.

Adult flies that are homozygous for esc⁶ and heterozygous for escl^d01514 are few and very weak. They display a range of homeotic phenotypes, including transformations of antenna to leg, wing to haltere and A4 to A5, as well as having extra sex combs on the leg in males.

(Ohno et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

19163

w¹¹¹⁸; P{XP}escl^d01514

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG16969^d01514

(Bloomington Drosophila Stock Center, 2004.11.6)

escl^d01514

(Margueron et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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