Pupal cold treatment (18[o]C) increases the average climb rates of kat-60L1^c01236 mutants to rates comparable to wild type controls.

kat-60L1^BE6 homozygotes and kat-60L1^BE6/kat-60L1^c01236 transheterozygotes exhibit a reduced lifespan, with 49% of transheterozygotes dying by 2 weeks after eclosion.

kat-60L1^BE6/kat-60L1^c01236 transheterozygous larvae exhibit a decreased response to noxious mechanical stimulus, exhibiting nocifensive escape locomotion approximately 40% of the time.

kat-60L1^BE6/kat-60L1^c01236 transheterozygous larvae are severely delayed in their response to a high-temperature probe of 46[o]C. This insensitivity is maintained at a lower threshold temperature of 42[o]C, indicating an overall failure to respond to noxious temperatures.

kat-60L1^BE6/kat-60L1^c01236 mutants exhibit large gaps in dorsal cluster dendritic arbors, both between neighbouring class IV dendritic arbours and within the arbour of an individual neuron. kat-60L1^BE6/kat-60L1^c01236 larvae exhibit a 26% reduction in grid squares containing dendrite segments, compared with control neurons. These flies exhibit a 22% reduction in the number of dendrite termini in class IV da neuron arbours. Average lengths of dendrite termini exhibit a 29% kat-60L1^BE6/kat-60L1^c01236 mutants compared to controls.

kat-60L1^BE6/kat-60L1^c01236 mutants do not exhibit any gross alterations in the appearance of commissures or the longitudinal axon tracts compared with controls.

At 48 hours AEL, kat-60L1^BE6/kat-60L1^c01236 mutants show slightly lower average dendritic coverage compared with controls, indicating a minor role for kat-60L1 in dendrite outgrowth before the scaling phase. Twenty-four hours later (72hrs AEL), during the first day of the third-instar stage. However, no significant difference in dendritic coverage is observed in mutant larvae compared with controls. Mutants are thus able to compensate for the earlier difference and establish full coverage, as well as maintain scaling growth, during this period. By the late wandering third-instar larval stage, however, kat-60L1^BE6/kat-60L1^c01236 mutants display on average only 69% dendritic coverage, compared to 93% in wild-type.

kat-60L1^BE6/kat-60L1^c01236 transheterozygous wandering, third instar larvae exhibit highly dynamic dendritic branching events. Terminal branches frequently extend, retract, or extend and retract within a 15-30 minute time period in kat-60L1^BE6/kat-60L1^c01236 mutants. The majority of branches are stable in control arbors, with only approximately 31% extending or retracting, while mutant arbors exhibit on average nearly twice as many dynamic branches (58%). Regardless of whether the dynamic events are extensions, retractions, or fluid events, all occur more frequently in mutant arbors compared to controls.

The dendritic morphology of ddaC neurons is normal in mutant larvae.

The proximal dendrites remain intact for many ddaC neurons at 16 hours after puparium formation (APF) in homozygous kat-60L1^c01236and kat-60L1^c01236/Df(3R)Exel6145 animals, indicating a defect in dendritic severing during dendrite pruning (proximal dendrites can be seen disconnected from the ddaC soma at 5 hours APF in wild-type animals).

The microtubules remain intact in the dendrites of kat-60L1^c01236 ddaC neurons at 5 hours after puparium formation (microtubules in the dendrites of wild-type ddaC neurons show breakage at this stage).

Homozygous mutant γ neuron clones in the mushroom body show normal axon pruning both at 18 hours APF and in the adult.