FB2026_02 , released June 18, 2026
Allele: Dmel\tefuwk
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General Information
Symbol
Dmel\tefuwk
Species
D. melanogaster
Name
FlyBase ID
FBal0175408
Feature type
allele
Associated gene
Dmel\tefu
Associated Insertion(s)
TI{TI}tefuwk
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
recombination
(Gong et al., 2005, Bi et al., 2004)
I-SceI
(Gong et al., 2005)
FLPase
(Gong et al., 2005)
ends-in gene targeting
(Bi et al., 2004)
gene targeting by homologous recombination
(Gong et al., 2005)
Progenitor genotype
Associated Insertion(s)
TI{TI}tefuwk
Cytology
Description

Duplication of the tefu locus. The 5' copy contains a small sequence insertion (caused by deletion of 13bp of endogenous tefu sequence and its replacement with 45bp that consists of part of the RS(I-SceI) restriction site and small repeats originating from the region adjacent to the restriction site) which results in a stop codon 15 codons downstream of the normal position of codon 902. The 3' copy contains stop codons in all six reading frames at the position corresponding to codon 195. A w+mW.hs marker and a single FRT site are located between the two copies of tefu.

(Gong et al., 2005)

Ends-in gene targeting event at the tefu locus.

(Bi et al., 2004)
Allele components
Component
Use(s)
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mutant larvae show a normal checkpoint response (a steep decline in the number of G2 cells entering mitosis) in response to irradiation with 4000 rad of X rays, but they are unable to block mitotic entry when irradiated with 500 rad.

      (Bi et al., 2005)

      Dividing neuroblasts in the brains of third instar mutant larvae show a number of defects. The most prominent defect is end-to-end association (telomere attachment) of chromosomes. Single telomere attachments (both between sister chromatids and between non-sister chromatids) and double telomere attachments are seen. In addition, chromosome breaks and aneuploid nuclei are seen. Chromosome bridges are often seen.

      (Bi et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      tefuwk has abnormal mitotic cell cycle phenotype, suppressible by DNAlig411/DNAlig411

      (Bi et al., 2004)
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      tefuwk has mitosis & nuclear chromosome phenotype, suppressible by DNAlig411/DNAlig411

      (Bi et al., 2004)

      tefuwk has chromosome, telomeric region phenotype, suppressible by DNAlig411/DNAlig411

      (Bi et al., 2004)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      tefuwk p53unspecified double mutants show the same checkpoint phenotype as tefuwk single mutants; they show a normal checkpoint response to irradiation with 4000 rad of X rays, but they are unable to block mitotic entry when irradiated with 500 rad.

      (Bi et al., 2005)

      The overall frequency of telomere attachments seen in tefuwk third instar larval neuroblasts is significantly reduced by Lig411/Lig411. Double telomere attachments drop most in frequency, while the frequency of single telomere attachments between sister chromatids is unaffected.

      (Bi et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Dp(3;3)tefuwk
      atmweak
      (Bi et al., 2004)
      atmwk
      (Bi et al., 2004)
      tefuwk
      (Gong et al., 2005)
      Name Synonyms
      Secondary FlyBase IDs
      • FBab0046724
      References (3)