FlyBase Allele Report: Dmel\DNAlig4[11]

General Information

Symbol

Dmel\DNAlig4¹¹

Species

D. melanogaster

Name

FlyBase ID

FBal0176090

Feature type

allele

Associated gene

Dmel\DNAlig4

Associated Insertion(s)

Carried in Construct

Also Known As

lig4¹¹

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Bi et al., 2004)

Progenitor genotype

DNAlig4^EP385

(Bi et al., 2004)

Cytology

Description

Imprecise excision of the P{EP}Lig4^EP385 element, resulting in a deletion of sequences encoding the first 147 codons of Lig4.

(Bi et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

chromosome

(Beaucher et al., 2012)

Detailed Description

Statement

Reference

Lig4¹¹ mutants have a significantly elevated frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 38^oC. Imperfect nonhomologous end joining (NHEJ) is significantly impaired compared to wild type under these conditions. Lig4¹¹ mutants do not show an increase in SSA frequency in the hemizygous assay when it is carried out at 32^oC.

Lig4¹¹ mutants mutants have an increased SSA frequency compared to controls in a P{wIw.FRT}/P{wIw.FRT.yellow} homozygous assay to study DNA double-stranded break repair when assayed at 38^oC.

(Wei and Rong, 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

DNAlig4¹¹/DNAlig4¹¹ is a suppressor of abnormal mitotic cell cycle phenotype of tefu^wk

(Bi et al., 2004)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

DNAlig4¹¹ is a non-enhancer of chromosome, telomeric region phenotype of woc²⁵¹

(Raffa et al., 2005)

Suppressor of

Statement

Reference

DNAlig4¹¹/DNAlig4¹¹ is a suppressor of mitosis & nuclear chromosome phenotype of tefu^wk

(Bi et al., 2004)

DNAlig4¹¹/DNAlig4¹¹ is a suppressor of chromosome, telomeric region phenotype of tefu^wk

(Bi et al., 2004)

NOT Suppressor of

Statement

Reference

DNAlig4¹¹ is a non-suppressor of chromosome, telomeric region phenotype of woc²⁵¹

(Raffa et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The frequency of telomere-telomere attachments seen in the brains of woc²⁵¹ Lig4¹¹ double mutant larvae is not significantly different from that found in woc²⁵¹ single mutants.

(Raffa et al., 2005)

The overall frequency of telomere attachments seen in tefu^wk third instar larval neuroblasts is significantly reduced by Lig4¹¹/Lig4¹¹. Double telomere attachments drop most in frequency, while the frequency of single telomere attachments between sister chromatids is unaffected.

(Bi et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

DNAlig4¹¹

Lig4^-

(Bi et al., 2004)

Lig4¹¹

(Beaucher et al., 2012)

lig4

(Raffa et al., 2005)

lig4¹¹

(Wei and Rong, 2007)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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