Expression of sra^GS3168 under the control of Scer\GAL4^elav-C155 or Scer\GAL4^sca-537.4 results in increased ovulation in virgin females compared to control virgin females. Within 30 minutes in a mating assay, only 35% of virgin females expressing sra^GS3168 under the control of Scer\GAL4^sca-537.4 have mated, compared to 82% of wild-type virgin females. Virgin females expressing sra^GS3168 under the control of Scer\GAL4^sca-537.4 elicit significantly lower courtship index (CI) levels (CI is the period of time within the observation period that a male displays any element of courtship behaviour) than wild-type virgin females. Virgin females expressing sra^GS3168 under the control of Scer\GAL4^sca-537.4 have a high extrusion index (EI, the percentage of a time a female shows extrusion behaviour when being courted by a male) which is nearly comparable to that of wild-type mated females. Homozygous females lay fewer eggs than wild type and most of the eggs are arrested at metaphase I of meiosis. Spontaneous ovulation occurs in 13% of mutant virgin females (virtually no ovulation occurs in wild-type virgin females). The level of ovulation of mutant mated females is not as high as that of wild-type mated females. The mutant females show reduced receptivity after mating (as occurs in wild-type females), but their remating rate 24 hours after mating is significantly higher than that of wild-type females. Receptivity of mutant females is restored earlier than for wild-type females; the remating rate is significantly higher for mutant females compared to wild-type females 10 days after mating.