FlyBase Allele Report: Dmel\Iqsec[T1032]

General Information

Symbol

Dmel\Iqsec^T1032

Species

D. melanogaster

Name

loner^T1032

FlyBase ID

FBal0175508

Feature type

allele

Associated gene

Dmel\Iqsec

Associated Insertion(s)

Carried in Construct

Also Known As

loner^T1032

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Chen et al., 2003)

Mutagen

ethyl methanesulfonate

(Chen et al., 2003)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C?T.

(Chen et al., 2003)

Numbering corresponds to RE02556 sequence.

(Chen et al., 2003)

Amino acid replacement: Q414term.

(Chen et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:21,070,924..21,070,924 [+]

Nucleotide change:

C21070924T

Reported nucleotide change:

C?T

Amino acid change:

Q402term | Iqsec-PA; Q414term | Iqsec-PB; Q299term | Iqsec-PC; Q309term | Iqsec-PD

Reported amino acid change:

Q414term

(Chen et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic myoblast

(Richardson et al., 2007, Chen et al., 2003)

fusion competent cell

(Bulchand et al., 2010)

Detailed Description

Statement

Reference

siz^T1032-homozygous clonal dorsal multidendritic ddaC neurons within a mosaic individual exhibit no defects in the dendritic arborization at the white pupa stage, nor in pruning at 16h after puparium formation, as compared to controls.

(Wang et al., 2017)

Myoblast fusion is blocked at the bi- and tri-nuclear stage in siz^T1032 mutant embryos.

(Bulchand et al., 2010)

siz^T1032 mutants exhibit increased numbers of wild-type sized actin foci, consistent with a block in myoblast fusion. Live imaging of siz^T1032 mutants indicates that whereas actin foci form normally, they never dissolve, correlating with the increased numbers of foci observed and a complete fusion block.

(Richardson et al., 2007)

In mutant embryos myoblast fusion fails to occur. Fusion competent myoblasts extend filopodia toward elongated founder cells, suggesting that adhesion between fusion-competent myoblasts and founder cells is not affected. Occasionally miniature fibres that contain two nuclei are seen.

(Chen et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Iqsec^T1032 has fusion competent cell phenotype, enhanceable by Df(3L)BK9

(Bulchand et al., 2010)

Iqsec^T1032 has fusion competent cell phenotype, enhanceable by Df(2R)Vrp1^D30

(Bulchand et al., 2010)

Iqsec^T1032 has fusion competent cell phenotype, enhanceable by blow²

(Bulchand et al., 2010)

Enhancer of

Statement

Reference

Iqsec^T1032/siz[+] is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2011)

Iqsec^T1032/siz[+] is an enhancer of retina phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

Iqsec^T1032/siz[+] is an enhancer of ommatidium phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

Iqsec^T1032/siz[+] is an enhancer of primary pigment cell phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

Iqsec^T1032/siz[+] is an enhancer of secondary pigment cell phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

Iqsec^T1032/siz[+] is an enhancer of tertiary pigment cell phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

Iqsec^T1032/siz[+] is an enhancer of cone cell phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

Iqsec^T1032/siz[+] is an enhancer of interommatidial precursor cell phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The eye mis-patterning phenotype resulting from the co-expression of Arf51F^GD13822 with Dcr-2^Scer\UAS.cDa under the control of Scer\GAL4^GMR.PF is significantly enhanced in siz^T1032 heterozygotes. The number of correctly patterned secondary and tertiary cell is reduced. The total number of interommatidial precursor cells (IPCs) does not differ markedly from the wild-type number of 12, indicating that the enhancement is due to specifically to increased disorder in IPC patterning. Additionally, errors in the placement of three bristle groups about each ommatidium and errors in primary pigment cell and cone cell patterning and ommatidial rotation are modestly enhanced.

The ommatidial patterning errors resulting from the expression of cindr^{dsRNA.PC.PD.Scer\UAS} via Scer\GAL4^GMR.PF are strongly enhanced in siz^T1032 heterozygotes.

(Johnson et al., 2011)

Myoblast fusion is completely blocked in Df(3L)BK9, siz^T1032 double mutant embryos. Founders remain mononucleate.

Myoblast fusion is significantly compromised in siz^T1032; Df(3L)BK9 double mutants. There is a greater percentage of hemisegments with a reduced number of nuclei in the double mutants than there is in the single mutants.

Myoblast fusion is significantly compromised in blow²; siz^T1032 double mutants. There is a greater percentage of hemisegments with a reduced number of nuclei in the double mutants than there is in the single mutants.

(Bulchand et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Iqsec^T1032

loner^T1032

(Wang et al., 2017, Deng et al., 2015, Bothe et al., 2014, Bulchand et al., 2010, Beckett and Baylies, 2007, Richardson et al., 2007, Onel et al., 2004)

siz^T1032

(Johnson et al., 2011)

Name Synonyms

lonerT1032

(Onel et al., 2004)

Secondary FlyBase IDs

References (9)

Report Sections

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