FlyBase Allele Report: Dmel\mre11[Δ35K1]

General Information

Symbol

Dmel\mre11^Δ35K1

Species

D. melanogaster

Name

FlyBase ID

FBal0175582

Feature type

allele

Associated gene

Dmel\mre11

Associated Insertion(s)

Carried in Construct

Also Known As

mre11^-

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Bi et al., 2004)

Mutagen

recombination

(Bi et al., 2004)

ends-out gene targeting

(Bi et al., 2004)

Progenitor genotype

Cytology

Description

Deletion of the entire mre11 coding region.

(Bi et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Oikemus et al., 2006, Bi et al., 2004)

abnormal mitotic cell cycle | third instar larval stage | conditional

(Yuan et al., 2018)

female sterile (with mre11^58S)

(Gao et al., 2009)

lethal - all die before end of pupal stage | recessive

(Bi et al., 2004)

radiation sensitive

(Bi et al., 2005)

some die during pupal stage | recessive

(Bi et al., 2004)

Phenotype Manifest In

chromosome, telomeric region

(Bi et al., 2004)

eye disc | third instar larval stage | conditional

(Yuan et al., 2018)

mitosis & nuclear chromosome

(Bi et al., 2004)

wing disc | third instar larval stage | conditional

(Yuan et al., 2018)

Detailed Description

Statement

Reference

mre11^Δ35K1 third instar larvae exposed to 10 Gy ionizing radiation exhibit a G2/M cell cycle checkpoint defect in wing and eye discs when compared to controls.

(Yuan et al., 2018)

Telomeric fusions in mre11^Δ35K1 mutants result in chromosome bridges during mitosis. Approximately 3.5% of mitotic nuclei taken from 11-s instar larvae display bridges, and 12.9% of mitotic nuclei taken from 6 third instar larvae do so. This suggests that the loss of the telomere protection function of maternal mre11 happens mainly during the third instar stage.

(Gao et al., 2009)

mre11^Δ35K1 homozygous or transheterozygous for mre11^Δ35K1/mre11^58S are viable and recovered at a Mendelian ratio.

(Gao et al., 2009)

mre11^Δ35K1 mutant cells exhibit DNA breaks and telomere fusions in metaphase and anaphase.

(Oikemus et al., 2006)

Mutant larvae show a normal checkpoint response (a steep decline in the number of G₂ cells entering mitosis) in response to irradiation with 4000 rad of X rays, but they are unable to block mitotic entry when irradiated with 500 rad. Mutant cells are highly defective in repairing chromosome breaks induced by X rays. Mutant nuclei irradiated with 500 rad display fragmented mitotic chromosomes beyond recognition.

(Bi et al., 2005)

Dividing neuroblasts in the brains of third instar mutant larvae show a number of defects. The most prominent defect is end-to-end association (telomere attachment) of chromosomes. Single telomere attachments (both between sister chromatids and between non-sister chromatids) and double telomere attachments are seen. In addition, chromosome breaks and aneuploid nuclei are seen. Chromosome bridges are often seen.

(Bi et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

mre11^Δ35K1, tefu^stg has lethal | recessive | larval stage phenotype

(Bi et al., 2004)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

mre11^Δ35K1 p53^unspecified double mutants show the same checkpoint phenotype as mre11^Δ35K1 single mutants; they show a normal checkpoint response to irradiation with 4000 rad of X rays, but they are unable to block mitotic entry when irradiated with 500 rad.

(Bi et al., 2005)

tefu^stg mre11^Δ35K1 double homozygotes die as late third instar larvae. Double mutants do not show an additive effect on the frequency of telomere attachments and chromosome breaks in third larval instar neuroblasts.

(Bi et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

The presence of mre11^58S rescues the viability of mre11^Δ35K1 homozygotes, although the surviving females are sterile.

(Gao et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

43639

mre11^Δ35K1/T(2;3)TSTL, CyO: TM6B, Tb⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

mre11^-

(Bi et al., 2005, Bi et al., 2004)

mre11^35K1

(Morciano et al., 2013)

mre11^Δ35K1

(Yuan et al., 2018, Gao et al., 2010, Gao et al., 2009, Gao et al., 2009)

mre11^Δ

(Oikemus et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

Open Close