FlyBase Allele Report: Dmel\RtGEF[p1036]

General Information

Symbol

Dmel\RtGEF^p1036

Species

D. melanogaster

Name

FlyBase ID

FBal0178146

Feature type

allele

Associated gene

Dmel\RtGEF

Associated Insertion(s)

P{EP}RtGEF^p1036

Carried in Construct

Also Known As

dPix^P1036

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Parnas et al., 2001)

Mutagen

Delta2-3 transposase

(Parnas et al., 2001)

Progenitor genotype

P{EP}CG16798^EP401

(Parnas et al., 2001)

Associated Insertion(s)

P{EP}RtGEF^p1036

Cytology

Description

P{EP} insertion into 5' part of rtGEF intron 1.

(Parnas et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with Df(2L)PJ19)

(Parnas et al., 2001)

abnormal neurophysiology (with Df(2L)PJ19)

(Parnas et al., 2001)

partially lethal - majority die (with Df(2L)PJ19)

(Parnas et al., 2001)

Phenotype Manifest In

NMJ bouton (with Df(2L)PJ19)

(Parnas et al., 2001)

postsynaptic membrane (with Df(2L)PJ19)

(Parnas et al., 2001)

synaptic vesicle (with Df(2L)PJ19)

(Parnas et al., 2001)

t-bar (with Df(2L)PJ19)

(Parnas et al., 2001)

Detailed Description

Statement

Reference

Homozygous clones generated using the eyFLP system do not result in any obvious overgrowth of the adult head.

(Dent et al., 2015)

rtGEF^p1036/Df(2L)PJ19 mutants lack the subsynaptic reticulum at the synapse of muscles 6 and 7 in third instar larvae. Additionally, these mutants have a greater number of T bars at the presynaptic active zone and there is an excess of vesicles in the terminal, which are larger than those of wild type, although vesicles in the mutant are able to dock at T bars. Further, boutons appear flatter than in wild type. Evoked excitatory junctional potentials (EJPs) are reduced and there is a greater variation in EJP amplitude per muscle in rtGEF^p1036/Df(2L)PJ19 mutants compared to wild type. While the amplitude of miniature EJPs is somewhat reduced in mutants, there is a large reduction in their frequency, indicating a presynaptic defect. Quantal content is slightly higher in rtGEF^p1036/Df(2L)PJ19 mutants than in wild type.

(Parnas et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

RtGEF^p1036, ft⁸ has visible | somatic clone phenotype

(Dent et al., 2015)

RtGEF^p1036, ft⁸ has partially lethal | somatic clone phenotype

(Dent et al., 2015)

RtGEF^p1036, ex^e1, ft⁸ has lethal | somatic clone phenotype

(Dent et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

RtGEF^p1036 is an enhancer of interommatidial cell | increased number | somatic clone phenotype of hpo^MGH1

(Dent et al., 2015)

Other

Statement

Reference

RtGEF^p1036, ft⁸ has ptilinum | somatic clone phenotype

(Dent et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Double homozygous ft⁸, RtGEF^p1036 clones generated using the eyFLP system result in substantial overgrowth of the head, particularly in the ptilinum.

The increase in the number of interommatidial cells which is seen in homozygous hpo^MGH1 clones generated using the eyFLP system is enhanced if the clones are also homozygous for RtGEF^p1036.

(Dent et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

RtGEF¹

(Parnas et al., 2001)

RtGEF²

(Parnas et al., 2001)

RtGEF³

(Parnas et al., 2001)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

RtGEF^p1036

dPix^P1036

(Lee and Schwarz, 2016)

dPix^p1036

(Ho and Treisman, 2020)

dpix¹⁰³⁶

(Bahri et al., 2009)

dpix^p1036

(Dent et al., 2019)

pix¹⁰³⁶

(Dent et al., 2015)

rtGEF^p1036

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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