A XhoI genomic fragment (+1594 to +6700bp relative to the ple transcription start site) has been replaced with a corresponding ple cDNA fragment, resulting in a minigene that lacks ple introns 2-6.
The embryonic lethality of ple mutants may be rescued by pleΔi2-6, but the degree of rescue varies from no rescue at all to a significant rescue level (80%) depending on the P{pleΔi2-6} insertion line used.