Construct: A XhoI fragment in pleΔi1-6 has been replaced with a corresponding ple genomic fragment (+1594 to +6700bp relative to the ple transcription start site), resulting in a minigene that lacks ple intron 1 but contains the other ple introns.
The embryonic lethality of ple mutants may be rescued by pleΔi1, but the degree of rescue varies from no rescue at all to a significant rescue level (80%) depending on the P{pleΔi1} insertion line used.