FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\bondZ5274
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General Information
Symbol
Dmel\bondZ5274
Species
D. melanogaster
Name
FlyBase ID
FBal0180718
Feature type
allele
Associated gene
Dmel\bond
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Szafer-Glusman et al., 2008, Giansanti et al., 2004)
Progenitor genotype
Cytology
Description

Disruption of a splicing signal, resulting in a frame shift at amino acid 83 followed by 3 extra amino acid residues and a stop codon.

(Szafer-Glusman et al., 2008)

Nucleotide substitution: G?A.

(Szafer-Glusman et al., 2008)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:22,555,014..22,555,014 [+]
Nucleotide change:

G22555014A

Reported nucleotide change:

G?A

Comment:

Mutation in splice donor site, resulting in a frame shift at amino acid 83 followed by 3 extra amino acid residues and a stop codon.

(Szafer-Glusman et al., 2008)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      contractile ring & meiotic cell cycle | male

      (Giansanti et al., 2004)

      Nebenkern

      (Giansanti et al., 2004)

      Nebenkern (with Df(3R)hh)

      (Giansanti et al., 2004)

      spermatid & nucleus | supernumerary

      (Giansanti et al., 2004)

      spermatid & nucleus | supernumerary (with Df(3R)hh)

      (Giansanti et al., 2004)

      spermatocyte (with bondZ3437)

      (Szafer-Glusman et al., 2008)

      spindle & meiotic cell cycle | male

      (Giansanti et al., 2004)
      Detailed Description
      Statement
      Reference

      bondZ5274 mutants exhibit male sterility with cytokinesis defects.

      (Chen et al., 2010)

      Constriction of the contractile ring is often minimal or slowed in bondZ5274/bondZ3437 spermatocytes, with these cells ultimately failing to complete cytokinesis. The contractile rings eventually detach from the plasma membrane in the mutant spermatocytes, with the cleavage furrow regressing rapidly and the poorly constricted ring constricting or collapsing rapidly and directionally from the site of membrane detachment towards the opposite site of the cell, where the ring remains attached to the cell cortex.

      (Szafer-Glusman et al., 2008)

      A large proportion of bondZ5274 mutant spermatids have one large nebenkern with four nuclei of normal size, indicating a cytokinetic failure in both meiotic divisions. A smaller proportion have two nuclei and a large nebenkern, indicating failure of only one of the meiotic divisions. The same phenotype is seen in bondZ5274/Df(3R)hh mutants. Spermatocytes of bondZ5274 mutants lack an organized central spindle and an F-actin ring throughout ana-telophase. The equatorial anillin band is present but fails to constrict properly.

      (Giansanti et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      bondZ5274 has male sterile phenotype, suppressible | partially by Pex2HP35039/CG7081[+]

      (Chen et al., 2010)

      bondZ5274 has abnormal cytokinesis phenotype, suppressible | partially by Pex2HP35039/CG7081[+]

      (Chen et al., 2010)

      bondZ5274 has male sterile phenotype, suppressible | partially by pex10[+]/Pex107

      (Chen et al., 2010)

      bondZ5274 has abnormal cytokinesis phenotype, suppressible | partially by pex10[+]/Pex107

      (Chen et al., 2010)

      bondZ5274 has male sterile phenotype, suppressible | partially by pex10[+]/Pex1010

      (Chen et al., 2010)

      bondZ5274 has abnormal cytokinesis phenotype, suppressible | partially by pex10[+]/Pex1010

      (Chen et al., 2010)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      A pex2HP35039/+ background partially suppresses the male sterility and cytokinesis defects found in bondZ5274 mutants. This is enhanced when in a pex107 or pex1010 background.

      A pex107/+ background partially suppresses the male sterility and cytokinesis defects found in bondZ5274 mutants. This is enhanced when in a pex2HP35039 background.

      A pex1010/+ background partially suppresses the male sterility and cytokinesis defects found in bondZ5274 mutants. This is enhanced when in a pex2HP35039 background.

      (Chen et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      bondZ5274
      (Chen et al., 2010, Szafer-Glusman et al., 2008)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)