FlyBase Allele Report: Dmel\hid[RNAi.hs]

General Information

Symbol

Dmel\hid^RNAi.hs

Species

D. melanogaster

Name

FlyBase ID

FBal0181049

Feature type

allele

Associated gene

Dmel\hid

Associated Insertion(s)

Carried in Construct

P{hs-hid.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Yin and Thummel, 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Yin and Thummel, 2004)

Mutagen

in vitro construct

(Yin and Thummel, 2004)

Progenitor genotype

Carried in construct

P{hs-hid.RNAi}

Cytology

Description

An Hsp70 promoter drives expression of a tandem inverted repeat of hid coding sequences.

(Yin and Thummel, 2004)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

hid

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death | cold sensitive

(Guha and Kornberg, 2005)

decreased cell death | heat sensitive

(Yin and Thummel, 2004)

Phenotype Manifest In

embryonic/larval salivary gland | P-stage | heat sensitive

(Yin and Thummel, 2004)

embryonic head | heat sensitive

(Yin and Thummel, 2004)

tracheal metamere 2 | cold sensitive

(Guha and Kornberg, 2005)

Detailed Description

Statement

Reference

Expression of W^dsRNA.hs, following a heat shock 0-2 hours after the L3 molt, disrupts the programmed death of larval cells in tracheal metamere 2.

(Guha and Kornberg, 2005)

Embryos expressing W^dsRNA.hs (using heat shock) have head involution defects (phenotype has 45% penetrance). 27% of animals in which W^dsRNA.hs is expressed using sequential 30 minute heat shock at 4 and 8 hours after puparium formation (APF) have persistent larval salivary glands at approximately 20 hours APF (the salivary glands have completed programmed cell death by this stage in wild-type animals). The penetrance of this phenotype is not altered if the animals also carry Df(3L)H99/+. Expression of W^dsRNA.hs in a wild-type background using sequential 30 minute heat shock at 8 and 4 hours before puparium formation does not have a significant effect on larval midgut cell death.

(Yin and Thummel, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

hid^RNAi.hs has decreased cell death | heat sensitive phenotype, enhanceable by Df(3L)H99/Df(3L)XR38

(Yin and Thummel, 2004)

Other

Statement

Reference

Df(3L)H99/Df(3L)XR38, hid^RNAi.hs has decreased cell death | heat sensitive phenotype

(Yin and Thummel, 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

hid^RNAi.hs has embryonic/larval salivary gland | P-stage | heat sensitive phenotype, enhanceable by Df(3L)H99/Df(3L)XR38

(Yin and Thummel, 2004)

Other

Statement

Reference

Df(3L)H99/Df(3L)XR38, hid^RNAi.hs has gastric caecum | P-stage | heat sensitive phenotype

(Yin and Thummel, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The percentage of animals having persistent larval salivary glands at 20 hours after puparium formation (APF) is increased from 27% in animals in which W^dsRNA.hs is expressed in a wild-type background using sequential 30 minute heat shock at 4 and 8 hours APF, to 65% in animals in which W^dsRNA.hs is expressed in a Df(3L)H99/Df(3L)XR38 background using sequential 30 minute heat shock at 4 and 8 hours APF. Expression of W^dsRNA.hs in a Df(3L)H99/Df(3L)XR38 background using sequential 30 minute heat shock at 8 and 4 hours before puparium formation has a significant effect on larval midgut cell death; 80% of these animals have persistent larval midguts which have failed to contract by 3 hours after puparium formation and retain their gastric caeca.

(Yin and Thummel, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

W^dsRNA.hs

hid^RNAi.hs

hid^dsRNA.hs

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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