FlyBase Allele Report: Dmel\Rab5[UAS.GFP]

General Information

Symbol

Dmel\Rab5^UAS.GFP

Species

D. melanogaster

Name

Rab5:GFP fusion construct of Shimizu

FlyBase ID

FBal0182040

Feature type

allele

Associated gene

Dmel\Rab5

Associated Insertion(s)

Carried in Construct

P{UAS-Rab5.GFP}

Also Known As

UAS-Rab5-GFP, UAS-GFP-Rab5

Key Links

Transgenic product class

wild_type

(Shimizu et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Shimizu et al., 2003)

Mutagen

in vitro construct

(Okajima, 2005, Shimizu et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Rab5.GFP}

Cytology

Description

UAS sequences drive expression of Rab5 tagged with GFP.

(Shimizu et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Rab5

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Rab5^UAS.GFP/Scer\GAL4^Toll-6-D42 is an enhancer of abnormal neurophysiology | third instar larval stage | dominant phenotype of Vps35^MH20

(Inoshita et al., 2017)

Suppressor of

Statement

Reference

Rab5^UAS.GFP/Scer\GAL4^da.G32 is a suppressor of abnormal size | third instar larval stage phenotype of Vps35^MH20/Vps35¹

(Inoshita et al., 2017)

Rab5^UAS.GFP/Scer\GAL4^ple.PF is a suppressor of abnormal circadian behavior | dominant | adult stage phenotype of Vps35¹

(Inoshita et al., 2017)

Rab5^UAS.GFP/Scer\GAL4^da.G32 is a suppressor of abnormal locomotor behavior | dominant | third instar larval stage phenotype of Vps35^MH20

(Inoshita et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

Rab5^UAS.GFP/Scer\GAL4^da.G32 is a suppressor of type I terminal bouton | third instar larval stage phenotype of Vps35^MH20/Vps35¹

(Inoshita et al., 2017)

Rab5^UAS.GFP/Scer\GAL4^da.G32 is a suppressor of synaptic vesicle | third instar larval stage phenotype of Vps35^MH20/Vps35¹

(Inoshita et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The expression of Rab5^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^da.G32 suppresses the reciprocal increased number of type Ib boutons and reciprocal decreased bouton size, and suppresses the reciprocal increased size and decreased number of synaptic vesicles and in the third instar larval neuromuscular junctions of Vps35¹/Vps35^MH20 transheterozygotes.

The expression of Rab5^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^ple.PF also suppresses the decreased sleep bouts displayed by Vps35¹ heterozygous adults.

The expression of Rab5^{Scer\UAS.T:Avic\GFP} suppresses the decreased locomotor activity (expression driven by Scer\GAL4^da.G32), while enhancing the decreased excitatory junction potential amplitude under high stimulation conditions at the neuromuscular junctions (expression driven by Scer\GAL4^Toll-6-D42), observed in Vps35^MH20 heterozygous third instar larvae.

(Inoshita et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Rab5^{Scer\UAS.T:Avic\GFP}

Rab5^UAS.GFP

Rab5^WT

(Takayama et al., 2014)

Name Synonyms

Rab5:GFP fusion construct of Shimizu

(Shimizu et al., 2003)

Secondary FlyBase IDs

References (7)

Report Sections

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