FlyBase Allele Report: Dmel\Fak[CG1]

General Information

Symbol

Dmel\Fak^CG1

Species

D. melanogaster

Name

FlyBase ID

FBal0183605

Feature type

allele

Associated gene

Dmel\Fak

Associated Insertion(s)

Carried in Construct

Also Known As

Fak56D^CG1, Fak56^CG1, DFak^CG1

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Murakami et al., 2007)

Mutagen

Delta2-3 transposase

(Grabbe et al., 2004)

Progenitor genotype

Fak^KG00304

(Grabbe et al., 2004)

Cytology

Description

Imprecise excision of the P{SUPor-P} element that removes the entire element and 1887bp of the Fak56D gene. This removes the first 1263bp of coding sequence, corresponding to the first 421 amino acids of Fak56D.

(Grabbe et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates tauopathy

modeled by Hsap\MAPT^4xA.UAS.2N4R

(Dourlen et al., 2017)

modeled by Hsap\MAPT^UAS.cWa

(Dourlen et al., 2017)

exacerbates multiple endocrine neoplasia type 2A

modeled by Ret^MEN2A.UAS

(Macagno et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

Fak^CG1 exacerbates the phenotypes seen in Ret^{MEN2A.Scer\UAS} when heterozygous with Fak^5-SZ-3124.

(Macagno et al., 2014)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult subretinal glial cell

(Murakami et al., 2007)

axon & eye photoreceptor cell

(Murakami et al., 2007)

bouton

(Chun-Jen Lin et al., 2011)

embryonic/larval optic stalk

(Murakami et al., 2007)

embryonic/larval optic stalk (with Df(2R)BSC26)

(Murakami et al., 2007)

neuromuscular junction | third instar larval stage

(Tsai et al., 2008)

synapse

(Chun-Jen Lin et al., 2011)

Detailed Description

Statement

Reference

Fak^CG1/+ larvae do not display increased dendrite crossing in the class IV dendrite arborizing neurons.

(Meltzer et al., 2016)

Eyes from Fak^CG1 null mutants display normal patterning.

(Macagno et al., 2014)

Synapse number is highly variable in Fak56D^CG1 mutant larvae. There is a tendency for synapse number to decrease, rather than increase, compared to wild-type.

Boutons in Fak56D^CG1 mutants often appear to be larger in size than wild-type.

Expression of Fak56D^{Scer\UAS.T:Ivir\HA1} in motor neurons under the control of Scer\GAL4^D42 in a Fak56D^CG1 background increases axon diameter.

Fak56D^CG1 larvae show an increased rate of onset of long term facilitation. EJP amplitude at 0.15mM and 0.2mM Ca[2+] concentrations is significantly increased but is not significantly different from wild-type amplitudes at 0.1mM Ca[2+] concentration in this mutant.

Miniature EJPs are unaffected in these mutants.

Expression of Fak56D^{Scer\UAS.T:Ivir\HA1} in motor neurons under the control of Scer\GAL4^D42 in a Fak56D^CG1 background does not affect miniature EJP amplitude.

(Chun-Jen Lin et al., 2011)

The neuromuscular junction is overgrown and shows an increase in bouton number in mutant third instar larvae.

(Tsai et al., 2008)

Photoreceptor axons fail to form a bundle at the optic stalk in homozygous mutants. In addition, the optic stalk itself is also severely disrupted, with about 80% of animals showing significant defects in optic stalk morphogenesis (the length of the stalk is less than or only equal to the diameter of the stalk, in contrast to the wild type where the length of the stalk is at least two times bigger than the diameter). These optic stalk defects are not detected until the early third larval instar and they become progressively more severe as development proceeds through the third larval instar.

Surface glia (SG) cells of the optic stalk fail to form a tubular structure in mutants, but instead spread over the surface of the optic lobe. This defect in CG cell localisation occurs before photoreceptor cell axon innervation. The SG cells show the same elongated morphology as wild type. However, analysis of small marked clones indicates that SG cells in mutant animals disperse along the anterior-posterior axis to a lesser extent than wild-type cells examined under the same conditions.

(Murakami et al., 2007)

Homozygous embryos show no obvious muscle disruption phenotypes compared to wild-type embryos. and no obvious defects in the migration of primordial midgut cells are seen.

(Grabbe et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Fak^CG1/Fak^5-SZ-3124 is an enhancer of partially lethal - majority die phenotype of Ret^MEN2A.UAS, Scer\GAL4^dpp.PU

(Macagno et al., 2014)

Fak^CG1/Fak[+] is an enhancer of visible phenotype of Ret^GMR.PR

(Macagno et al., 2014)

Fak^CG1 is an enhancer of visible phenotype of Ret^GMR.PR

(Macagno et al., 2014)

NOT Enhancer of

Statement

Reference

Fak^CG1, Fak^UAS.Tag:HA, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Ret^GMR.PR

(Macagno et al., 2014)

Fak^CG1, Fak^Y430F.UAS, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Ret^GMR.PR

(Macagno et al., 2014)

Suppressor of

Statement

Reference

Fak^CG1 is a suppressor of abnormal neuroanatomy phenotype of CaMKII^T287D.UAS, Scer\GAL4^Toll-6-D42

(Chun-Jen Lin et al., 2011)

Other

Statement

Reference

Fak^CG1, p130CAS¹ has lethal | embryonic stage phenotype

(Tikhmyanova et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Fak^CG1 has embryonic/larval optic stalk phenotype, enhanceable by CdGAPr^NP3053/CdGAPr[+]

(Murakami et al., 2007)

Fak^CG1 has embryonic/larval optic stalk phenotype, enhanceable by CdGAPr[+]/CdGAPr^EY13451

(Murakami et al., 2007)

Fak^CG1 has embryonic/larval optic stalk phenotype, enhanceable by mys¹/mys[+]

(Murakami et al., 2007)

Fak^CG1 has embryonic/larval optic stalk phenotype, enhanceable by mys¹¹/mys[+]

(Murakami et al., 2007)

Enhancer of

Statement

Reference

Fak^CG1/Fak^5-SZ-3124 is an enhancer of wing disc phenotype of Ret^MEN2A.UAS, Scer\GAL4^dpp.PU

(Macagno et al., 2014)

Fak^CG1/Fak[+] is an enhancer of eye phenotype of Ret^GMR.PR

(Macagno et al., 2014)

Fak^CG1 is an enhancer of eye phenotype of Ret^GMR.PR

(Macagno et al., 2014)

Fak^CG1, Fak^UAS.Tag:HA, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Ret^GMR.PR

(Macagno et al., 2014)

Fak^CG1/Fak56D[+] is an enhancer of embryonic/larval optic stalk phenotype of mys¹

(Murakami et al., 2007)

Fak^CG1/Fak56D[+] is an enhancer of embryonic/larval optic stalk phenotype of mys¹¹

(Murakami et al., 2007)

Fak^CG1/Fak56D[+] is an enhancer of embryonic/larval optic stalk phenotype of CdGAPr^NP3053

(Murakami et al., 2007)

Fak^CG1/Fak56D[+] is an enhancer of embryonic/larval optic stalk phenotype of CdGAPr^EY13451

(Murakami et al., 2007)

NOT Enhancer of

Statement

Reference

Fak^CG1 is a non-enhancer of motor neuron phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^Toll-6-D42

(Chun-Jen Lin et al., 2011)

Fak^CG1 is a non-enhancer of synapse phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^Toll-6-D42

(Chun-Jen Lin et al., 2011)

Fak^CG1 is a non-enhancer of axon phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^Toll-6-D42

(Chun-Jen Lin et al., 2011)

Suppressor of

Statement

Reference

Fak^CG1 is a suppressor of synapse phenotype of CaMKII^T287D.UAS, Scer\GAL4^Toll-6-D42

(Chun-Jen Lin et al., 2011)

Fak^CG1 is a suppressor of axon phenotype of CaMKII^T287D.UAS, Scer\GAL4^Toll-6-D42

(Chun-Jen Lin et al., 2011)

Fak^CG1 is a suppressor of motor neuron phenotype of CaMKII^T287D.UAS, Scer\GAL4^Toll-6-D42

(Chun-Jen Lin et al., 2011)

NOT Suppressor of

Statement

Reference

Fak^CG1 is a non-suppressor of synapse phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^Toll-6-D42

(Chun-Jen Lin et al., 2011)

Fak^CG1 is a non-suppressor of axon phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^Toll-6-D42

(Chun-Jen Lin et al., 2011)

Fak^CG1 is a non-suppressor of motor neuron phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^Toll-6-D42

(Chun-Jen Lin et al., 2011)

Fak^CG1/Fak56D[+] is a non-suppressor of eye phenotype of Atg1^K.GMR

(Chen et al., 2008)

Other

Statement

Reference

Fak^CG1, Ret^GMR.PR has ommatidium phenotype

(Macagno et al., 2014)

Fak^CG1, Ret^GMR.PR has eye disc phenotype

(Macagno et al., 2014)

Fak^CG1, Ret^GMR.PR has retina phenotype

(Macagno et al., 2014)

Fak^CG1, Ret^GMR.PR has interommatidial cell phenotype

(Macagno et al., 2014)

Fak^CG1, Ret^GMR.PR has cone cell | increased number | pupal stage phenotype

(Macagno et al., 2014)

Fak^CG1, p130CAS¹ has embryo | late embryonic stage phenotype

(Tikhmyanova et al., 2010)

Fak^CG1, p130CAS¹ has larva | first instar larval stage phenotype

(Tikhmyanova et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Fak^CG1,Df(2R)Sema-2b-C4 double mutant larvae do not display increased dendrite crossing in the class IV dendrite arborizing neurons.

(Meltzer et al., 2016)

Heterozygous Fak^CG1 enhances the ommatidial mis-patterning seen in eyes expressing Ret^GMR.PR. Homozygous Fak^CG1 causes a smaller eye due to a completely disrupted ommatidial pattern. This enhancement is reversed upon co-expression of either Fak^{Scer\UAS.T:Ivir\HA1} or Fak^{Y430F.Scer\UAS} under the control of Scer\GAL4^GMR.PU.

The retinas of pupae (42 hours after pupal formation) expressing Ret^GMR.PR in a homozygous Fak^CG1 mutant background lack the hexagonal array and identifiable ommatidial units. Numerous ct-positive cone cells are seen. Some bristle cells and a few cells recognisable as primary pigment cells remain, but there are no detectable cells with the appearance of normal interommatidial cells. Ommatidial units with extranumerary cone cells are also seen at early stages of development (4 hours after pupal development). Eye discs contain normal number of photoreceptors, albeit some clusters have rotation defects.

Fak^CG1/Fak^5-SZ-3124 enhances the partial lethality and wing disc defects seen when Ret^{MEN2A.Scer\UAS} is expressed under the control of Scer\GAL4^dpp.PU. The proportion of surviving flies rises from 46% to 92%.

(Macagno et al., 2014)

A Fak56D^CG1 background completely suppresses the increased synapse number conferred by CaMKII^{T287D.Scer\UAS} expression. Synapse number in these double mutants is indistinguishable from that in Fak56D^CG1 single mutants.

The increase in synapse number seen in larvae expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} in motor neurons under the control of Scer\GAL4^D42 is unaffected by a Fak56D^CG1 background.

The axon diameter increase seen upon expression of CaMKII^{T287D.Scer\UAS} under the control of Scer\GAL4^D42 is suppressed by a Fak56D^CG1 mutant background.

The axon diameter increase seen upon expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} under the control of Scer\GAL4^D42 is unaffected by a Fak56D^CG1 mutant background.

(Chun-Jen Lin et al., 2011)

Fak56D^CG1/+; p130CAS¹/Df(3L)Exel6083 adults do not emerge, indicating complete lethality. Analysis of the Fak56D^CG1/Fak56D^CG1; p130CAS¹/+ lethal phenotype indicates that most (95%) of the embryos do not hatch. The few escapers survive to pupal stages, but do not emerge. Only 5% of Fak56D^CG1; p130CAS¹ double homozygotes produce cuticles and almost all are marked by dorsal and/or ventral holes, indicating dorsal closure defects. These cuticles also show fused or missing denticle belts.

(Tikhmyanova et al., 2010)

CdGAPr^NP3053 Fak56D^CG1 double heterozygotes show severe defects in optic stalk morphology, in contrast to either single heterozygote, which show only slight defects in optic stalk formation.

CdGAPr^EY13451 Fak56D^CG1 double heterozygotes show severe defects in optic stalk morphology, in contrast to either single heterozygote, which show only slight defects in optic stalk formation.

Fak56D^CG1 mys¹ double heterozygotes show more severe defects in optic stalk morphology than either single heterozygote, which each show only slight defects in optic stalk formation.

Fak56D^CG1 mys¹¹ double heterozygotes show more severe defects in optic stalk morphology than either single heterozygote, which each show only slight defects in optic stalk formation.

(Murakami et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Fak^CG1 is rescued by Fak^UAS.Tag:HA/Scer\GAL4^Toll-6-D42

(Chun-Jen Lin et al., 2011)

Fak^CG1 is rescued by Scer\GAL4^NP4702/Fak^UAS.Tag:HA

(Murakami et al., 2007)

Partially rescued by

Fak^CG1 is partially rescued by Fak^UAS.Tag:HA/Scer\GAL4^NP2109

(Murakami et al., 2007)

Not rescued by

Fak^CG1 is not rescued by Fak^UAS.Tag:HA/Scer\GAL4^GMR.PY

(Murakami et al., 2007)

Comments

Expression of Fak56D^{Scer\UAS.T:Ivir\HA1} in motor neurons under the control of Scer\GAL4^D42 restores normal glutamate sensitivity to Fak56D^CG1 mutants.

Expression of Fak56D^{Scer\UAS.T:Ivir\HA1} in motor neurons under the control of Scer\GAL4^D42 restores wild-type levels of long term facilitation in Fak56D^CG1 mutants.

The increased EJP amplitude found in Fak56D^CG1 mutants is rescued by motor neuron-dependent expression of Fak56D^{Scer\UAS.T:Ivir\HA1}.

(Chun-Jen Lin et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

600230

w^*; Fak^CG1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

DFak^CG1

(Chun-Jen Lin et al., 2011)

Fak56^CG1

(Tsai et al., 2008, Grabbe et al., 2004)

Fak56D^CG1

(Tikhmyanova et al., 2010, Chen et al., 2008, Murakami et al., 2007)

Fak^CG-1

(Jin et al., 2020)

Fak^CG1

(Dourlen et al., 2017, Kim et al., 2014, Chen et al., 2008)

Fak^CG

(Green et al., 2018)

dFAK^CG1

(Macagno et al., 2014)

fak56^CG1

(Meltzer et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (12)

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