Approximately 17% of Dlc90F^e155/Df(3R)Exel6178 prophase spermatocytes have free centrosomes (unattached to the cortex or nuclear surface) compared to only ~3% in wild type. In ~14% of spermatocytes the centrosomes remain anchored at the cortex rather than at the nuclear surface as is seen in wild type.

Nucleus-basal body uncoupling is seen in Dlc90F^e155/Df(3R)Exel6178 mutant hemizygous spermatids. Nebenkern-basal body attachments and nucleus-nebenkern attachments are also frequently lost. Furthermore, the Nebenkern occasionally display abnormal morphology.

After six days away from females, the testes of wild-type males become swollen. Such swelling does not occur in Dlc90F^e155 males and their testes do not contain accumulated motile sperm in the seminal vesicles. Early onion stage Dlc90F^e155 spermatids have elongated flagella, but sperm tails are bundled less tightly than those of wild type and coiling of the tails is less compact. The nuclei of Dlc90F^e155 spermatids are scattered along the entire length of the cyst, instead of being bundled at the end, as in wild type. The mispositioned mutant nuclei frequently fail to completely elongate. Those nuclei that localize normally at the basal end of the cyst elongate normally. Mislocalized nuclei are not associated with a flagellar basal body, whereas the few nuclei that localize correctly in the cysts of Dlc90F^e155 mutants are localized with a basal body. Dlc90F^e155 sperm individualization appears normal. Unlike its vertebrate homologue, Dlc90F is not required for retina formation and function as this is unaffected in Dlc90F^e155 mutants.