FlyBase Allele Report: Dmel\CadN[7b-13a-18a.UAS]

General Information

Symbol

Dmel\CadN^{7b-13a-18a.UAS}

Species

D. melanogaster

Name

FlyBase ID

FBal0184427

Feature type

allele

Associated gene

Dmel\CadN

Associated Insertion(s)

Carried in Construct

P{UAS-CadN.7b-13a-18a}

Key Links

Transgenic product class

wild_type

(Ting et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ting et al., 2005)

Mutagen

in vitro construct

(Ting et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-CadN.7b-13a-18a}

Cytology

Description

UASt regulatory sequences drive expression of a CadN isoform which contains the alternative exons 7b, 13a and 18a.

(Ting et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

CadN

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size | larval stage, with Scer\GAL4^how-24B

(Hsu et al., 2009)

abnormal locomotor behavior | larval stage, with Scer\GAL4^how-24B

(Hsu et al., 2009)

lethal - all die during larval stage, with Scer\GAL4^how-24B

(Hsu et al., 2009)

sterile, with CadN⁴⁰⁵/CadN^M19, Scer\GAL4^elav.PU

(Hsu et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Scer\GAL4^how-24B-mediated expression of CadN^{7b-13a-18a.Scer\UAS} does not affect the survival rate during embryogenesis, but induces robust (100%) lethality during larval stages. Larvae exhibit reduced locomotion and remain small in size prior to death.

Scer\GAL4^elav.PU-mediated expression of CadN^{7b-13a-18a.Scer\UAS} induces no abnormality through both the embryonic and larval stages.

(Hsu et al., 2009)

Wild-type R cell clones that express CadN^{7b-13a-18a.Scer\UAS}, under the control of Scer\GAL4^elav-C155, show normal axon extension.

(Prakash et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^elav.PU/CadN^{7b-13a-18a.UAS} partially rescues CadN⁴⁰⁵/CadN^M19

(Hsu et al., 2009)

Scer\GAL4^elav-C155/CadN^{7b-13a-18a.UAS} partially rescues Df(2L)CadN^Δ14

(Prakash et al., 2005)

Scer\GAL4^elav-C155/CadN^{7b-13a-18a.UAS} partially rescues CadN⁴⁰⁵

(Ting et al., 2005)

Fails to rescue

Scer\GAL4^how-24B/CadN^{7b-13a-18a.UAS} fails to rescue CadN⁴⁰⁵/CadN^M19

(Hsu et al., 2009)

Comments

Scer\GAL4^how-24B-mediated expression of CadN^{7b-13a-18a.Scer\UAS} has no effect on the lethality of CadN^M19/CadN⁴⁰⁵.

Scer\GAL4^elav.PU-mediated expression of CadN^{7b-13a-18a.Scer\UAS} partially rescues the lethality of CadN^M19/CadN⁴⁰⁵ - animals now survive through to the adult stage, but fail to produce progeny.

(Hsu et al., 2009)

Expression of CadN^{7b-13a-18a.Scer\UAS}, under the control of Scer\GAL4^elav-C155 in Df(2L)CadN^Δ14 R-cell clones, results in ommatidia in which 49% of R cells show normal axon extension, compared to 100% of R cells showing abnormal extension in Df(2L)CadN^Δ14 animals not expressing the transgene.

(Prakash et al., 2005)

The mistargeting and growth cone morphology defects seen in CadN⁴⁰⁵ mutant R7 cells at 17% and 35% APF are substantially rescued by expression of CadN^{7b-13a-18a.Scer\UAS} under the control of Scer\GAL4^elav-C155.

(Ting et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CadN^{7b-13a-18a.Scer\UAS}

CadN^{7b-13a-18a.UAS}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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