FlyBase Allele Report: Dmel\CadN[405]

General Information

Symbol

Dmel\CadN⁴⁰⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0124374

Feature type

allele

Associated gene

Dmel\CadN

Associated Insertion(s)

Carried in Construct

Also Known As

Ncad⁴⁰⁵

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous embryos show mild central nervous system defects.

(Biersmith et al., 2011)

Proximal arborization is occasionally eliminated in neuron clones homozygous for CadN⁴⁰⁵. The defect is adult brains are diverse.

(Hasegawa et al., 2011)

In CadN⁴⁰⁵ mutant homozygous clones, R7 cells terminate their axon projections in the outer M3 layer, rather than the M6 layer.

(Petrovic and Hummel, 2008)

At 48% after pupal formation (APF), all R cell axons homozygous for CadN⁴⁰⁵ extend aberrantly (64%), or do not extend at all (36%).

(Nern et al., 2005)

At 17% APF, 21% of CadN⁴⁰⁵ R7 growth cones (analysed as single cell mutant clones) fail to reach the R7-temporary layer in the medulla; the mutant axons either expand their growth cones incorrectly at the R8-temporary layer or between the R7 and R8 temporary layers. In addition, over half of the mutant growth cones show severe morphological defects. At 25% APF, 55% of the mutant R7 axons terminate at the R8 temporary layer or between the R7 and R8 temporary laters. Some of the mutant axons leave a small filopodium connecting to the R7 temporary layer.

(Ting et al., 2005)

Mosaic flies in which the photoreceptor cells are homozygous show defects in the optomotor response.

(Clandinin et al., 2001)

The ability of mutant flies to detect motion is approximately 5 times worse than wild-type. The response of mutant flies to a UV/Vis choice test is approximately 8 fold worse than wild-type. Mutants perform well, though less well than wild-type, in counter-current fast phototaxis assays. In CadN⁴⁰⁵ mosaic eyes, the R7 and R8 projections that normally form a regular array in the medulla fail to form this array and often terminate at the outer edge of the medulla. Individual R cells do not make the characteristic projections seen in the wild-type. In the optic lobe of CadN⁴⁰⁵ mosaic eyes, the lamina plexus forms irregular clumps, rather than the smooth line of uniform thickness seen in wild-type. R8 termini exhibit defects in local topographical mapping failing to form the regular array in the medulla normally seen. The array of R4 termini is also disrupted, also R4 growth cones do not expand fully within the lamina plexus. The array of R7 termini is also disrupted and their termini exhibit elongated thickening along the terminal region. However ganglion specificity is not affected and the columnar organisation of the lamina neurons is normal. Glial cells differentiate and migrate normally into the lamina, however the organisation of glial cells is disrupted such that in some regions of the lamina, they fail to form the three layers seen in wild-type. When individual R7 axons are mutant, they fail to terminate at the R7 target layer, M6, and stop instead at the R8 layer, M3. However topographic mapping of the R7 in the medulla appears normal in these animals. When homozygous somatic clones are made in the eye so that only mutant R7 cells persist, the response from these flies to a UV/Vis choice test is several times worse than wild-type.

(Lee et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

CadN⁴⁰⁵ has abnormal neuroanatomy phenotype, non-suppressible by seq^UAS.cBa/Scer\GAL4^GMR.long

(Petrovic and Hummel, 2008)

Enhancer of

Statement

Reference

CadN[+]/CadN⁴⁰⁵ is an enhancer of abnormal neuroanatomy | somatic clone phenotype of Rich²

(Tong et al., 2011)

NOT Enhancer of

Statement

Reference

CadN[+]/CadN⁴⁰⁵ is a non-enhancer of abnormal neuroanatomy | somatic clone phenotype of Rich¹

(Tong et al., 2011)

Suppressor of

Statement

Reference

CadN⁴⁰⁵ is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^GMR.long, seq^UAS.cBa

(Petrovic and Hummel, 2008)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

CadN⁴⁰⁵ has photoreceptor cell R7 phenotype, non-suppressible by seq^UAS.cBa/Scer\GAL4^GMR.long

(Petrovic and Hummel, 2008)

CadN⁴⁰⁵ has medulla layer M6 phenotype, non-suppressible by seq^UAS.cBa/Scer\GAL4^GMR.long

(Petrovic and Hummel, 2008)

CadN⁴⁰⁵ has medulla layer M3 phenotype, non-suppressible by seq^UAS.cBa/Scer\GAL4^GMR.long

(Petrovic and Hummel, 2008)

Enhancer of

Statement

Reference

CadN[+]/CadN⁴⁰⁵ is an enhancer of photoreceptor cell R7 | somatic clone phenotype of Rich²

(Tong et al., 2011)

NOT Enhancer of

Statement

Reference

CadN[+]/CadN⁴⁰⁵ is a non-enhancer of photoreceptor cell R7 | somatic clone phenotype of Rich¹

(Tong et al., 2011)

Suppressor of

Statement

Reference

CadN⁴⁰⁵ is a suppressor of photoreceptor cell R8 phenotype of Scer\GAL4^GMR.long, seq^UAS.cBa

(Petrovic and Hummel, 2008)

CadN⁴⁰⁵ is a suppressor of medulla layer M6 phenotype of Scer\GAL4^GMR.long, seq^UAS.cBa

(Petrovic and Hummel, 2008)

CadN⁴⁰⁵ is a suppressor of medulla layer M3 phenotype of Scer\GAL4^GMR.long, seq^UAS.cBa

(Petrovic and Hummel, 2008)

Additional Comments

Genetic Interactions

Statement

Reference

CadN⁴⁰⁵/+ enhances the R7 targeting phenotype seen in Rich² eye clones made using the 'eyFLP' system.

CadN⁴⁰⁵/+ does not enhance the R7 targeting phenotype seen in Rich¹ eye clones made using the 'eyFLP' system.

(Tong et al., 2011)

CadN⁴⁰⁵ suppresses the R8 cell targeting defects seen when seq^Scer\UAS.cBa is expressed under the control of Scer\GAL4^GMR.long. The R8 axons no longer terminate in the M6 layer of the medulla and correctly target the M3 layer. However the flies do show an R7 targeting phenotype; the R7 photoreceptors target the M3 layer rather than the M6 layer, as is seen in CadN⁴⁰⁵ mutants alone.

(Petrovic and Hummel, 2008)

CadN⁴⁰⁵ Lar²¹²⁷ double mutant R7 growth cones show similar phenotypes to CadN⁴⁰⁵ single mutant R7 growth cones.

(Ting et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

CadN⁴⁰⁵/CadN^M19 is partially rescued by Scer\GAL4^elav.PU/CadN^{7b-13a-18a.UAS}

(Hsu et al., 2009)

CadN⁴⁰⁵/CadN^M19 is partially rescued by Scer\GAL4^elav.PU/CadN^{7b-13b-18a.UAS}

(Hsu et al., 2009)

CadN⁴⁰⁵ is partially rescued by Scer\GAL4^Tub.PU/CadN^18B.UAS

(Nern et al., 2005)

CadN⁴⁰⁵ is partially rescued by CadN^18A.UAS/Scer\GAL4^Tub.PU

(Nern et al., 2005)

CadN⁴⁰⁵ is partially rescued by Scer\GAL4^Tub.PU/CadN^UAS.cIa

(Nern et al., 2005)

CadN⁴⁰⁵ is partially rescued by Scer\GAL4^elav-C155/CadN^{7b-13a-18a.UAS}

(Ting et al., 2005)

CadN⁴⁰⁵ is partially rescued by Scer\GAL4^elav-C155/CadN^{7b-13b-18a.UAS}

(Ting et al., 2005)

CadN⁴⁰⁵ is partially rescued by Scer\GAL4^elav-C155/CadN^{7b-13a-18b.UAS}

(Ting et al., 2005)

CadN⁴⁰⁵ is partially rescued by Scer\GAL4^elav-C155/CadN^UAS.cIa

(Ting et al., 2005)

Not rescued by

CadN⁴⁰⁵/CadN^M19 is not rescued by Scer\GAL4^how-24B/CadN^{7b-13a-18a.UAS}

(Hsu et al., 2009)

CadN⁴⁰⁵/CadN^M19 is not rescued by Scer\GAL4^how-24B/CadN^{7b-13b-18a.UAS}

(Hsu et al., 2009)

Comments

Scer\GAL4^how-24B-mediated expression of CadN^{7b-13a-18a.Scer\UAS} has no effect on the lethality of CadN^M19/CadN⁴⁰⁵.

Scer\GAL4^elav.PU-mediated expression of CadN^{7b-13a-18a.Scer\UAS} partially rescues the lethality of CadN^M19/CadN⁴⁰⁵ - animals now survive through to the adult stage, but fail to produce progeny.

(Hsu et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Found in a screen using a retina dependant behavioural test - an optomotor assay to assess the function of photoreceptor cells R1 to R6.

(Lee et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CadN⁴⁰⁵

(Kulkarni et al., 2016, Tong et al., 2011, Petrovic and Hummel, 2008)

N-cad⁴⁰⁵

(Hummel and Zipursky, 2004)

NCad^omb405

(Hasegawa et al., 2011)

Ncad1^omb405

(Biersmith et al., 2011)

Ncad⁴⁰⁵

(Biersmith et al., 2011, Hsu et al., 2009, Ting et al., 2005, Clandinin et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (11)

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