Open Close
General Information
Symbol
Dmel\seqUAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Brenman
FlyBase ID
FBal0178045
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-seq
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of wild type seq gene is governed by UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Over-expression of seqScer\UAS.cBa in a wild-type CNS under the control of Scer\GAL4elav-C155; Scer\GAL4sca-109-68 results in the abrogation of ganglionic branching.

The growth cones seen in the axons of R8/R7 photoreceptor cells of pupal stage flies expressing seqScer\UAS.cBa under the control of Scer\GAL4GMR.long fail to undergo the morphological change from an expanded to a condensed appearance. R8 axons extend towards and terminate precisely in the R7 layer (M6), resulting in a single R8/R7 layer in M6 in the medulla of adult flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Simultaneous expression of seqScer\UAS.cBa and bnlScer\UAS.cSa in the CNS under the control of Scer\GAL41407 has the same effect as over-expression of bnlScer\UAS.cSa alone.

Expression of seqScer\UAS.cBa under the control of Scer\GAL4GMR.long induces the R7 photoreceptor cells in sev3 mutants to switch from targeting the M3 layer of the medulla to the M6 layer.

CadN405 suppresses the R8 cell targeting defects seen when seqScer\UAS.cBa is expressed under the control of Scer\GAL4GMR.long. The R8 axons no longer terminate in the M6 layer of the medulla and correctly target the M3 layer. However the flies do show an R7 targeting phenotype; the R7 photoreceptors target the M3 layer rather than the M6 layer, as is seen in CadN405 mutants alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of seqScer\UAS.cBa in the ectoderm under the control of Scer\GAL469B rescues the ganglionic branch bifurcation phenotype found in seqC022 and seqZ1241 homozygotes and seqZ1241/seqC022 trans-heterozygotes.

Expression of seqScer\UAS.cBa in midline precursors and glia of seqZ1241, seqC022 and seqZ1241/seqC022 mutants under the control of Scer\GAL4sim.PS accomplishes approximately 50% rescue with those embryos that were not fully rescued showing a considerable attenuation of the phenotype.

Expression of seqScer\UAS.cBa under the control of Scer\GAL4GMR.long rescues the R7/R8 photoreceptor targeting defects seen when sequnspecified mutant somatic clones are induced in R7 cells.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
seqScer\UAS.cBa
seqUAS.cBa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Brenman
Secondary FlyBase IDs
    References (7)