FlyBase Allele Report: Dmel\bnl[UAS.cSa]

Ectopic expression of bnl^Scer\UAS.cSa in somatic clones of tracheal cells redirects tracheal progenitor migration. Dual clones induce bifurcation with groups of progenitors mowing toward each ectopic bnl^Scer\UAS.cSa source. Clones in Tr3 and posterior metameres cause progenitors in these regions to leave the niche, even though they do not normally do so. When bnl^Scer\UAS.cSa-expressing clones fail to induce migration, the clones appear to be too far from the progenitors or there is competition from another clone close by.

(Chen and Krasnow, 2014)

In the CNS, bnl^Scer\UAS.cSa expression in the midline using Scer\GAL4^sim.PS results in the abrogation of ganglionic branching. Higher levels of expression, under the control of Scer\GAL4¹⁴⁰⁷ cause a more extreme phenotype, with many cells adopting terminal fate.

(Araújo and Casanova, 2011)

Expression of bnl^Scer\UAS.cSa under the control of Scer\GAL4^69B has little or no effect on mesoderm monolayer formation in the embryo following gastrulation.

(Kadam et al., 2009)

When bnl^Scer\UAS.cSa is driven by Scer\GAL4^bap.3 about a 1/4 of visceral mesoderm parasegments, visceral branches bifurcate, in about 2.4% they show simple misrouting. These defects persist until at least stage 15.

(Hosono et al., 2003)

Ectopic expression of bnl^Scer\UAS.cSa under the control of Scer\GAL4^Act5C.PP in clones in the male genital disc results in the migration of btl-expressing cells into a nearby ectopic domain where they are not observed in wild-type discs.

(Ahmad and Baker, 2002)

Tracheal cells of all types in bnl^Scer\UAS.cSa; Scer\GAL4^btl.PS embryos have numerous ectopic filpodia.

(Ribeiro et al., 2002)

In late third instar bnl^Scer\UAS.cSa; Scer\GAL4^dpp.blk1 larvae ectopic tracheoblasts can be seen adhering to the disc in the vicinity of the dpp expression domain. When somatic clones of bnl^Scer\UAS.cSa; Scer\GAL4^Act5C.PI cells are randomly induced in wing discs, ectopic tracheoblasts migrate along the surface of disc towards these clones.

(Sato and Kornberg, 2002)

Ectopic cellular extensions are seen in the tracheal cells of stage 12 embryos expressing bnl^Scer\UAS.cSa under the control of Scer\GAL4^btl.PS.

(Wolf et al., 2002)

Embryos expressing bnl^Scer\UAS.cSa under the control of Scer\GAL4^btl.PS develop complete dorsal trunk structures but lack the other primary branches.

(Wolf and Schuh, 2000)

Branching of the dorsal branch and lateral trunk of the tracheal system is suppressed in embryos expressing bnl^Scer\UAS.cSa under the control of Scer\GAL4^btl.PS and the dorsal trunk appears thickened.

(Ikeya and Hayashi, 1999)

Ubiquitous expression of bnl^Scer\UAS.cSa driven by Scer\GAL4^hs.PB and heatshocked for 1 hour during the first larval instar, causes a dramatic proliferation of terminal branches. However, unlike normal terminal branches, which are distributed evenly over their targets, these remain clumped together in a large tangled mass near the point of origin. Over expression of bnl^Scer\UAS.cSa when driven by Scer\GAL4^A9 in the CNS, transforms the normal ladder-like pattern of GB tracheal branches into a massive tangle of branches that completely cover the CNS. When driven by Scer\GAL4^hs.PB, a similar effect is seen in the LG trachea. A similar effect is seen when expressed in most tissues, but the heart and imaginal discs, which normally express bnl but lack tracheal branches, fail to attract branches even when the gene is overexpressed there. When bnl^Scer\UAS.cSa is driven in the salivary glands (which do not normally express bnl or receive trachea) by Scer\GAL4^71B during larval development, terminal tracheal branches are attracted to the salivary glands from nearby tissues. When bnl^Scer\UAS.cSa is driven in muscle fibre 12 by Scer\GAL4^5053A the LG terminal branches are directed towards this muscle, away from their normal targets at the ventral midline, doubling the number seen terminating on this muscle (as compared to wild-type) when raised at 25^oC and quadrupling the number when raised at 29^oC. Expression of bnl^Scer\UAS.cSa in random single cells (Driven by Scer\GAL4^Act5C.PP in a Scer\FLP1^hs.PS induced clones.), causes a local proliferation of terminal branches that grow out to the expressing cells arborizing on the cells and almost completely engulfing them. Attraction occurs over distances of up to about 10 cell diameters. Cases can be found where a terminal branch bifurcates to target two bnl^Scer\UAS.cSa expressing cells separated by several cell diameters.

(Jarecki et al., 1999)

Expression of bnl^Scer\UAS.cSa under the control of Scer\GAL4^69B results in marked defects in tracheal branching; primary branching is suppressed and an overabundance of secondary and terminal branches is induced.

(Lin et al., 1999)

When expression is driven at stage 11 by heat induced Scer\GAL4^hs.PB the number of cells expression pantip and terminal markers is increased in all tracheal branches. Extra terminal branching occurs.

(Steneberg et al., 1999)

Expression of bnl^Scer\UAS.cSa under the control of Scer\GAL4^69B results in a reduction of primary branching and an increase in the formation of fine secondary and terminal branches in the embryonic tracheal system.

(Michelson et al., 1998)

Scer\GAL4^C49, Scer\GAL4^69B or Scer\GAL4^hs.PB mediated expression in wild type branches causes a disruption in the normal pattern of branching; a mass of fine branches growing out in random directions from stunted branches.

(Sutherland et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^sim.PS, bnl^UAS.cSa has tracheal section phenotype, enhanceable by sr^UAS.cBa

(Dorfman et al., 2002)

Scer\GAL4^btl.PS, bnl^UAS.cSa has embryonic/larval tracheal dorsal trunk phenotype, enhanceable by hb¹⁵

(Wolf and Schuh, 2000)

Suppressed by

Statement

Reference

Scer\GAL4^69B, bnl^UAS.cSa has larval tracheal system phenotype, suppressible | partially by sfl⁰³⁸⁴⁴

(Lin et al., 1999)

Scer\GAL4^69B, bnl^UAS.cSa has larval tracheal system phenotype, suppressible | partially by sgl⁰⁸³¹⁰

(Lin et al., 1999)

Scer\GAL4^69B, bnl^UAS.cSa has larval tracheal system phenotype, suppressible by stumps^YY202

(Michelson et al., 1998)

NOT suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, bnl^UAS.cSa has embryonic/larval ganglionic tracheal branch phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/seq^UAS.cBa

(Araújo and Casanova, 2011)

Enhancer of

Statement

Reference

Scer\GAL4^btl.PS/bnl^UAS.cSa is an enhancer of embryonic/larval tracheal dorsal trunk phenotype of hb¹⁵

(Wolf and Schuh, 2000)

Suppressor of

Statement

Reference

Scer\GAL4^twi.PG/bnl^UAS.cSa is a suppressor of larval tracheal system phenotype of stumps^09904b

(Imam et al., 1999)

bnl^UAS.cSa/Scer\GAL4^69B is a suppressor of larval tracheal system phenotype of sfl⁰³⁸⁴⁴

(Lin et al., 1999)

bnl^UAS.cSa/Scer\GAL4^69B is a suppressor of larval tracheal system phenotype of sgl⁰⁸³¹⁰

(Lin et al., 1999)

NOT Suppressor of

Statement

Reference

bnl^UAS.cSa/Scer\GAL4^69B is a non-suppressor of tracheal primordium | embryonic stage phenotype of fand^fas-1/fand^H124

(Sauerwald et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The lack of primary tracheal branching characteristic for fand^fas-1/fand^H124 transheterozygous embryos cannot be rescued by expression of bnl^Scer\UAS.cSa under the control of Scer\GAL4^69B (which on its own causes excessive tracheal branching).

(Sauerwald et al., 2017)

Simultaneous expression of seq^Scer\UAS.cBa and bnl^Scer\UAS.cSa in the CNS under the control of Scer\GAL4¹⁴⁰⁷ has the same effect as over-expression of bnl^Scer\UAS.cSa alone.

(Araújo and Casanova, 2011)

When sr^Scer\UAS.cBa and bnl^Scer\UAS.cSa are both driven by Scer\GAL4^sim.PS a dramatic tracheal sprouting is seen in the salivary glands, showing an enhancement of the bnl^Scer\UAS.cSa, Scer\GAL4^sim.PS phenotype.

(Dorfman et al., 2002)

hb¹⁵ mutant embryos that express bnl^Scer\UAS.cSa ectopically have no signs of dorsal trunk outgrowth at all.

(Wolf and Schuh, 2000)

When expression is driven in the mesoderm of a stumps^09904b mutant by Scer\GAL4^twi.PG, the portions of the tracheal sacs near the bnl^Scer\UAS.cSa expressing cells significant cytoplasmic outgrowth and ramification of terminal branches. Some erratic of primary branches also occurs. These features are not normally seen in stumps^09904b mutants. Similar effects are seen when bnl^Scer\UAS.cSa is expressed under the control of Scer\GAL4^twi.PG in Df(3R)γ2 homozygotes.

(Imam et al., 1999)

The tracheal branching defects caused by expression of bnl^Scer\UAS.cSa under the control of Scer\GAL4^69B are weakly blocked if the embryos are homozygous for sfl⁰³⁸⁴⁴ or sgl⁰⁸³¹⁰. Partially restores tracheal branching in sfl⁰³⁸⁴⁴ embryos derived from sfl⁰³⁸⁴⁴ female germline clones (lacking both maternal and zygotic sfl function), when expressed under the control of Scer\GAL4^69B. Partially restores tracheal branching in sgl⁰⁸³¹⁰ embryos derived from sgl⁰⁸³¹⁰ female germline clones (lacking both maternal and zygotic sgl function), when expressed under the control of Scer\GAL4^69B.

(Lin et al., 1999)

The tracheal phenotype caused by expression of bnl^Scer\UAS.cSa under the control of Scer\GAL4^69B is strongly suppressed by stumps^YY202.

(Michelson et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

bnl^UAS.cSa/Scer\GAL4^Hand.Δ rescues bnl^GD3070

(Destalminil-Letourneau et al., 2021)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

64232

w^*; P{UAS-bnl.S}A12/CyO; MKRS/TM6B, Tb¹

64231

w^*; P{AyGAL4}25, P{UAS-EGFP}5a.2; P{UAS-bnl.S}A1-1/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

bnl^Scer\UAS.cSa

bnl^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Sutherland

(Sutherland et al., 1996)

Secondary FlyBase IDs

References (37)

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