FB2026_02 , released June 18, 2026
Allele: Dmel\fandH124
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General Information
Symbol
Dmel\fandH124
Species
D. melanogaster
Name
FlyBase ID
FBal0325716
Feature type
allele
Associated gene
Dmel\fand
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Sauerwald et al., 2017)
Progenitor genotype
Cytology
Description

Amino acid replacement: W219term.

(Sauerwald et al., 2017)

Nucleotide substitution: G?A.

(Sauerwald et al., 2017)

Predicted to result in a truncated protein lacking 11 of the 13 tetratricopeptide repeats of the fand protein.

(Sauerwald et al., 2017)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:13,594,439..13,594,439 [+]
Nucleotide change:

G13594439A

Reported nucleotide change:

G?A

Amino acid change:

W219term | fand-PA

Reported amino acid change:

W219term

Comment:

Nonsense mutation (TGG to TAG) at Trp codon.

(Sauerwald et al., 2017)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      fandH124 homozygotes are embryonic lethal and display defects in primary tracheal branching as the branch outgrowth is severely impaired, adjacent metameres often form partially connected dorsal trunk, earlier processes - gastrulation and germband retraction appear unaffected, however the mutant embryos show defects in later stages of embryogenesis, including abnormal dorsal closure and head involution. Defective tracheal branching is also observed in fandP218/fandH124 transheterozygous embryos.

      fandfas-1/fandH124 transheterozygous embryos show defective morphogenesis of the tracheal as well as the central nervous system.

      (Sauerwald et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The tracheal branching defects characteristic for fandH124 mutant embryos are not noticeably improved by expression of CG17716Scer\UAS.cSa in the ectoderm under the control of an unspecified Gal4 driver.

      The lack of primary tracheal branching characteristic for fandfas-1/fandH124 transheterozygous embryos cannot be rescued by expression of bnlScer\UAS.cSa under the control of Scer\GAL469B (which on its own causes excessive tracheal branching).

      (Sauerwald et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      fandfas-1/fandH124 is rescued by fand+tGa

      (Sauerwald et al., 2017)

      fandfas-1/fandH124 is rescued by Scer\GAL4da.PU/fandUASp.Tag:MYC

      (Sauerwald et al., 2017)

      fandfas-1/fandH124 is rescued by fandUASp.Tag:MYC/Scer\GAL4Abd-B-LDN

      (Sauerwald et al., 2017)
      Partially rescued by

      fandfas-1/fandH124 is partially rescued by Scer\GAL4da.PU/fandUASp.Tag:MYC/Scer\GAL80btl

      (Sauerwald et al., 2017)
      Not rescued by

      fandfas-1/fandH124 is not rescued by Scer\GAL4btl.PS/fandUASp.Tag:MYC

      (Sauerwald et al., 2017)
      Comments

      The fand05488, fandfas-1, fand1 as well as fand2 alleles each fails to complement both fandP218 and fandH124 alleles.

      The tracheal branching and central nervous defects characteristic for fandfas-1/fandH124 embryos are fully rescued by combination with a single copy of fand+tGa. The lack of tracheal branching is also rescued by expression of fandScer\UAS.T:Hsap\MYC when driven by Scer\GAL4da.PU or Scer\GAL4Abd-B-LDN and partially so when driven by Scer\GAL4da.PU combined with Scer\GAL80btl (to block the expression in tracheal cells), while it cannot be rescued by Scer\GAL4btl.PS-driven expression.

      (Sauerwald et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      fandH124
      fasH124
      (Sauerwald et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)