FlyBase Allele Report: Dmel\trem[f05981]

General Information

Symbol

Dmel\trem^f05981

Species

D. melanogaster

Name

FlyBase ID

FBal0185623

Feature type

allele

Associated gene

Dmel\trem

Associated Insertion(s)

PBac{WH}trem^f05981

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

piggyBac activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Lake et al., 2011)

Mutagen

piggyBac activity

(Gene Disruption Project members and Exelixis, 2005)

Progenitor genotype

Associated Insertion(s)

PBac{WH}trem^f05981

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{WH}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:20,031,266..20,031,267 [+]

(Lake et al., 2011, Lake et al., 2007, Page et al., 2007, Gene Disruption Project members and Exelixis, 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle (with trem¹²⁷²)

(Lake et al., 2011)

abnormal meiotic cell cycle (with trem³¹⁰⁰)

(Lake et al., 2011)

abnormal meiotic cell cycle (with trem^F9)

(Lake et al., 2011)

abnormal meiotic cell cycle | recessive

(Lake et al., 2011)

female semi-sterile

(Lake et al., 2011)

increased cell death

(Lake et al., 2011)

Phenotype Manifest In

ovary

(Lake et al., 2011)

Detailed Description

Statement

Reference

The few progeny produced by trem^f05981 mutant females exhibit high levels of nondisjunction. The number of meiotically-induced double stranded breaks seen in pro-oocytes in region 2A of the germarium is reduced compared to wild type. This reduction is likely due to initiation of DSB formation, as DSBs are can be induced when the mutants are treated with X-rays.

Prior to stage 6 the ovaries of trem^f05981 mutant females appear relatively normal, showing normal DNA morphology and specification of the oocyte. By the time the females are 2 days old almost no late stage egg chambers are detected. The ovaries appear very rudimentary and show evidence of apoptosis. No fertility defects are observed in trem^f05981 mutant males. The fertility defects are not improved upon treatment with X-rays.

trem^F9/trem^f05981 mutant females exhibit increased nondisjunction and a reduction in meiotic recombination.

trem¹²⁷²/trem^f05981 mutant females exhibit increased nondisjunction and a reduction in meiotic recombination. The number of meiotically-induced double stranded breaks is reduced compared to wild type.

trem³¹⁰⁰/trem^f05981 mutant females exhibit increased nondisjunction and a reduction in meiotic recombination. The number of meiotically-induced double stranded breaks (DSBs) is reduced compared to wild type.

(Lake et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

trem^f05981 is a suppressor of abnormal meiotic cell cycle phenotype of okr^AA/okr^RU

(Lake et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

trem^f05981 is a suppressor | partially of karyosome phenotype of okr^AA/okr^RU

(Lake et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The karyosome defects observed in okr^AA/okr^RU are almost completely suppressed in the background of trem^f05981.

trem^f05981 suppresses the accumulation of meiotically-induced double stranded breaks seen in region 3 of the germarium in okr^AA/okr^RU mutant females.

(Lake et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

trem^F9

(Lake et al., 2007)

Rescued by

trem^F9/trem^f05981 is rescued by Scer\GAL4^{VP16.nanos.UTR}, trem^f05981

(Lake et al., 2011)

trem^f05981 is rescued by Scer\GAL4^{VP16.nanos.UTR}, trem^f05981

(Lake et al., 2011)

trem^f05981 is rescued by trem^+t2.7

(Lake et al., 2011)

Partially rescued by

trem^f05981 is partially rescued by trem^P299L

(Lake et al., 2011)

trem^f05981 is partially rescued by trem^P327L

(Lake et al., 2011)

trem^f05981 is partially rescued by trem^P355L

(Lake et al., 2011)

trem^f05981 is partially rescued by trem^P383L

(Lake et al., 2011)

trem^f05981 is partially rescued by trem^H294Y

(Lake et al., 2011)

trem^f05981 is partially rescued by trem^H406Y

(Lake et al., 2011)

Not rescued by

trem^f05981 is not rescued by trem^H322Y

(Lake et al., 2011)

trem^f05981 is not rescued by trem^H350Y

(Lake et al., 2011)

trem^f05981 is not rescued by trem^H378Y

(Lake et al., 2011)

trem^f05981 is not rescued by trem^ZAD1

(Lake et al., 2011)

trem^f05981 is not rescued by trem^ZAD2

(Lake et al., 2011)

Rescues

Scer\GAL4^{VP16.nanos.UTR}, trem^f05981 rescues trem^F9/trem^f05981

(Lake et al., 2011)

Scer\GAL4^{VP16.nanos.UTR}, trem^f05981 rescues trem^f05981

(Lake et al., 2011)

Comments

Expression of trem^f05981 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the fertility defects seen in the trem^f05981 mutant females.

Expression from the UAS element in the trem^f05981 insertion under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the increase in nondisjunction seen in the trem^F9/trem^f05981 mutant females.

Expression of trem^+t2.7 rescues the fertility defects seen in trem^f05981 mutant females.

Expression of trem^P299L rescues the fertility but not the meiotic defects observed in trem^f05981 homozygotes.

Expression of trem^P327L rescues the fertility but not the meiotic defects observed in trem^f05981 homozygotes.

Expression of trem^P355L rescues the fertility but not the meiotic defects observed in trem^f05981 homozygotes.

Expression of trem^P383L rescues the fertility but not the meiotic defects observed in trem^f05981 homozygotes.

Expression of trem^H294Y rescues the fertility but not the meiotic defects observed in trem^f05981 homozygotes.

Expression of trem^H322Y does not rescue either the fertility or the meiotic defects observed in trem^f05981 homozygotes.

Expression of trem^H350Y does not rescue either the fertility or the meiotic defects observed in trem^f05981 homozygotes.

Expression of trem^H378Y does not rescue either the fertility or the meiotic defects observed in trem^f05981 homozygotes.

Expression of trem^H406Y rescues the fertility but not the meiotic defects observed in trem^f05981 homozygotes.

Expression of trem^ZAD1 does not rescue the fertility or defects observed in trem^f05981 homozygotes.

Expression of trem^ZAD2 does not rescue the fertility or defects observed in trem^f05981 homozygotes.

(Lake et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

85711

w¹¹¹⁸; PBac{WH}trem^f05981

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG4413^f05981

trem^f05981

(Lake et al., 2011, Page et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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