Plp⁵ homozygous or Plp⁵/Df(3L)BSC441 transheterozygous stage 16 embryos exhibit a small but significantly higher frequency of nuclear positioning defects within myotubes than controls (i.e. nucleus clusters traverse less of the distance toward the muscle poles).

cp309⁵/Df(3L)Brd15 central brain neuroblasts show a general disorganization of pericentriolar material throughout mitosis, and centrosomes are much more disorganized than controls.

cp309⁵ mutant third instar larval brains exhibit defects in recruitment to the centrosome of γTub23C, γTub37C, cnn, tacc, and Map60 proteins in early mitosis. The size and distribution of centrioles in wild-type and cp309⁵ mutant interphase larval brain cells is indistinguishable. The same is true for testes cells. cp309⁵ brain cells do not exhibit a higher mitotic index than control cells. In cp309⁵ mutant testes, morphologically normal primary spermatocytes are formed that each contain two pairs of large, orthogonally arranged centrioles. However, as the spermatocytes mature, the centrioles often lose their orthogonal arrangement and partially fragment. cp309⁵ mutant spermatocytes often form multipolar meiosis I spindles, with each spindle pole organised by at least one centriole. Although meiosis is highly abnormal in cp309⁵ mutant spermatocytes, at least some cells develop into relatively normal looking sperm. However, the distribution of centrioles and nuclei in the cysts is often disorganised. Moreover, although the cp309⁵ mutant sperm contain flagella, these are often nonmotile, even though electron microscopy reveals the sperm tails to be structurally normal.

Plp⁵ has nucleus | embryonic stage 16 phenotype, non-enhanceable by Nin^null.R/Nin^null.R

Plp⁵ has embryonic somatic muscle cell | embryonic stage 16 phenotype, non-enhanceable by Nin^null.R/Nin^null.R