FlyBase Allele Report: Dmel\Nin[null.R]

General Information

Symbol

Dmel\Nin^null.R

Species

D. melanogaster

Name

FlyBase ID

FBal0346145

Feature type

allele

Associated gene

Dmel\Nin

Associated Insertion(s)

TI{mCherry}Nin^null.R

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

gene targeting by homologous recombination

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Rosen et al., 2019)

Mutagen

gene targeting by homologous recombination

(Rosen et al., 2019)

Progenitor genotype

Associated Insertion(s)

TI{mCherry}Nin^null.R

Cytology

Description

All Bsg25D protein coding sequence has been deleted. The following sequence (derived from the pTV[Cherry]-based donor) has been inserted in place of the deleted Bsg25D sequence: a single attP site and a loxP cassette which itself contains an mFRT71 cassette followed by Scer\GAL4 coding sequence and a polyadenylation signal (this GAL4 sequence is non-functional). The mFRT71 cassette contains 1: the mCherry coding region tagged with a membrane-targeting signal (Tag:Myr(Unk)) and followed by a polyadenylation signal, 2: a w^+* marker.

(Rosen et al., 2019)

Allele components

Component

Use(s)

Inserted element

TI{mCherry}

promoter trap

Encoded product / tool

mCherry

red fluorescent protein

Also carries

attP

integrase target site

loxP

recombinase target site

mFRT71

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

2L:5,272,630..5,278,214 [-]

Comment:

The Bsg25D protein sequence has been deleted and replaced with a pTV[Cherry]-based sequence (see allele description).

(Rosen et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | larval stage

(Rosen et al., 2019)

fertile

(Rosen et al., 2019)

partially lethal - majority live

(Rosen et al., 2019)

some die during larval stage

(Rosen et al., 2019)

some die during pupal stage

(Rosen et al., 2019)

Phenotype Manifest In

Detailed Description

Statement

Reference

Bsg25D^null.R maternal-zygotic mutants show a modest decrease in survival to adulthood compared to controls but adults are fertile; third instar larvae crawl lower distances than controls.

Bsg25D^null.R homozygous stage 16 embryo myotubes exhibit apparently normal nuclear spread and positioning; the microtubule organization is apparently normal. The same is observed in larval muscles.

(Rosen et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Nin^null.R, ens[+]/ens^swo has some die during embryonic stage | maternal effect phenotype

(Rosen et al., 2019)

Nin^null.R, ens[+]/ens^swo has majority die during embryonic stage | maternal effect phenotype

(Rosen et al., 2019)

Nin^null.R, ens[+]/ens^swo has lethal - all die before end of larval stage | maternal effect phenotype

(Rosen et al., 2019)

Nin^null.R/Df(2L)BSC693, ens[+]/ens^swo has some die during embryonic stage | maternal effect phenotype

(Rosen et al., 2019)

Nin^null.R/Df(2L)BSC693, ens[+]/ens^swo has majority die during embryonic stage | maternal effect phenotype

(Rosen et al., 2019)

Nin^null.R/Df(2L)BSC693, ens[+]/ens^swo has lethal - all die before end of larval stage | maternal effect phenotype

(Rosen et al., 2019)

Nin^null.R/Dp(2;3)GV-CH321-49G22, ens[+]/ens^swo has viable phenotype

(Rosen et al., 2019)

Phenotype Manifest In

Suppressed by

Statement

Reference

Nin^null.R, ens^swo has nucleus | embryonic stage 16 phenotype, suppressible by Dp(2;3)GV-CH321-49G22/+

(Rosen et al., 2019)

NOT Enhancer of

Statement

Reference

Nin^null.R/Nin^null.R is a non-enhancer of nucleus | embryonic stage 16 phenotype of Plp⁵

(Rosen et al., 2019)

Nin^null.R/Nin^null.R is a non-enhancer of embryonic somatic muscle cell | embryonic stage 16 phenotype of Plp⁵

(Rosen et al., 2019)

Nin^null.R/Df(2L)BSC693 is a non-enhancer of nucleus | embryonic stage 16 phenotype of ens^swo

(Rosen et al., 2019)

Nin^null.R/Df(2L)BSC693 is a non-enhancer of embryonic somatic muscle cell | embryonic stage 16 phenotype of ens^swo

(Rosen et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

The progeny from the cross between Bsg25D^null.R/Bsg25D^null.R fathers and either Bsg25D^null.R/Bsg25D^null.R, ens^swo/+ or Bsg25D^null.R/Df(2L)BSC693, ens^swo/+ mothers is embryonic or larval lethal; progeny from the reciprocal cross does not show any major viability defects, as compared to controls. The lethality of the former is rescued by one copy of Dp(2;3)GV-CH321-49G22.

Bsg25D^null.R/Bsg25D^null.R, ens^swo/+ and Bsg25D^null.R/Df(2L)BSC693, ens^swo/+ stage 16 embryos exhibit a small but significantly higher frequency of nucleus positioning defects in myotubes as compared to ens^swo/+ individuals: nucleus clusters traverse less of the distance toward the muscle poles; in some myotubes, myonuclei are present as one cluster. The nucleus positioning defects of the former individuals are partially rescued by one copy of Dp(2;3)GV-CH321-49G22.

Bsg25D^null.R, ens^swo double homozygous stage 16 embryos exhibit the same severe myotube nucleus clustering as observed in ens^swo single homozygotes.

Both Bsg25D^null.R/Bsg25D^null.R, Khc⁸/+ and Bsg25D^null.R/Bsg25D^null.R, Dhc64C^4-19/+ stage 16 embryo myotubes present normal nuclear positioning.

(Rosen et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Bsg25D^null.R

Bsg25D^null

(Rosen et al., 2019)

Nin^null.R

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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