FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\locoP283
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General Information
Symbol
Dmel\locoP283
Species
D. melanogaster
Name
FlyBase ID
FBal0190811
Feature type
allele
Associated gene
Dmel\loco
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Yu et al., 2005)
Mutagen
Delta2-3 transposase
(Yu et al., 2005)
Progenitor genotype
locoEY04589
(Yu et al., 2005)
Cytology
Description

Imprecise excision of locoEY04589 generates locoP283, in which nucleotides -310 to +2195 of the transcript (start point of loco-c1 transcript is +1) are depleted, removing the RGS domain, two RBD domains, and the GoLoco motif.

(Yu et al., 2005)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      locoP283 homozygotes are viable and display severe locomotion defects. locoP283 embryos, lacking both maternal and zygotic components exhibit defects in mitotic spindle orientation. In cells of mitotic domain 9, the mitotic spindle that normally rotates by 90o to align along the apical/basal axis in wild-type often fails to re-orientate. The vast majority of locoP283 mutant neuroblasts, lacking both maternal and zygotic components, divide asymmetrically to produce daughters of different sizes as in wild-type neuroblasts. However, a small proportion (~10%) of locoP283 neuroblasts undergo similar-sized division.

      (Yu et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      locoP283 has abnormal neuroanatomy phenotype, enhanceable by pinsunspecified

      (Yu et al., 2005)
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      locoP283 has astral microtubule & neuroblast | ectopic phenotype, enhanceable by pinsunspecified

      (Yu et al., 2005)

      locoP283 has neuroblast phenotype, enhanceable by pinsunspecified

      (Yu et al., 2005)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      In embryos derived from double germline clones of locoP283 and rapsunspecified, the majority of neuroblasts undergo symmetric divisions to generate two similar-sized daughter cells in stage 10 mutant embryos, in 60% of cases. The cleavage plane is placed near the middle of the two centrosomes and the spindle is positioned symmetrically with both centrosomes lying in close proximity to the cell cortex. Astral microtubules, which are normally associated only with the apical centrosome in wild-type neuroblasts, can emanate from both centrosomes in locoP283 and rapsunspecified double mutant neuroblasts.

      (Yu et al., 2005)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      locoP283
      (Kopp and Park, 2019)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)