FlyBase Allele Report: Dmel\mib1[ΔRF.UAS.Tag:polyHis,Tag:MYC]

General Information

Symbol

Dmel\mib1^{ΔRF.UAS.Tag:polyHis,Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0191342

Feature type

allele

Associated gene

Dmel\mib1

Associated Insertion(s)

Carried in Construct

P{UAS-mib1.ΔRF}

Key Links

Transgenic product class

inframe_deletion

(Lai et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Lai et al., 2005)

Mutagen

in vitro construct

(Lai et al., 2005)

Progenitor genotype

mib1^{UAS.Tag:polyHis,Tag:MYC}

(Lai et al., 2005)

Carried in construct

P{UAS-mib1.ΔRF}

Cytology

Description

The C-terminal most RING finger domain of mib1 has been deleted.

(Lai et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tagged with

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult head bristle, with Scer\GAL4^sca-537.4

(Lai et al., 2005)

campaniform sensillum | increased number, with Scer\GAL4^dpp.blk1

(Lai et al., 2005)

eye disc, with Scer\GAL4^unspecified

(Lai et al., 2005)

sensory mother cell | increased number, with Scer\GAL4^sca-537.4

(Lai et al., 2005)

wing, with Scer\GAL4^Bx-MS1096

(Lai et al., 2005)

wing disc, with Scer\GAL4^dpp.blk1

(Lai et al., 2005)

wing margin, with Scer\GAL4^Bx-MS1096

(Lai et al., 2005)

wing margin, with Scer\GAL4^dpp.blk1

(Lai et al., 2005)

wing vein, with Scer\GAL4^Bx-MS1096

(Lai et al., 2005)

wing vein L3, with Scer\GAL4^dpp.blk1

(Lai et al., 2005)

Detailed Description

Statement

Reference

Expression of mib1^{ΔRF.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC}, under the control of Scer\GAL4^sca-537.4, induces mild bristle tufting on the head. Third instar wing imaginal discs of this genotype show ectopic L3 sensory precursors. Flies that express mib1^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 have vestigial wings that completely lack a wing margin; the remaining wing tissue present is composed mostly of severely thickened wing veins. In flies that express mib1^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} under the control of Scer\GAL4^dpp.blk1 the wing displays an extremely thickened L3 vein and a vast surplus of campaniform sensilla. Wings show a large gap in the wing margin. Expression of mib1^{ΔRF.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} inhibits eye disc growth.

(Lai et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^dpp.blk1, mib1^{ΔRF.UAS.Tag:polyHis,Tag:MYC} has campaniform sensillum phenotype, suppressible | partially by neur^UAS.cLa/Scer\GAL4^dpp.blk1

(Lai et al., 2005)

Scer\GAL4^dpp.blk1, mib1^{ΔRF.UAS.Tag:polyHis,Tag:MYC} has wing margin phenotype, suppressible | partially by neur^UAS.cLa/Scer\GAL4^dpp.blk1

(Lai et al., 2005)

Scer\GAL4^dpp.blk1, mib1^{ΔRF.UAS.Tag:polyHis,Tag:MYC} has wing vein L3 phenotype, suppressible | partially by neur^UAS.cLa/Scer\GAL4^dpp.blk1

(Lai et al., 2005)

NOT suppressed by

Statement

Reference

Scer\GAL4^dpp.blk1, mib1^{ΔRF.UAS.Tag:polyHis,Tag:MYC} has wing disc phenotype, non-suppressible by Delta^UAS.cDa, Scer\GAL4^dpp.blk1

(Lai et al., 2005)

Suppressor of

Statement

Reference

mib1^{ΔRF.UAS.Tag:polyHis,Tag:MYC}/Scer\GAL4^dpp.blk1 is a suppressor of wing disc | ventral phenotype of Scer\GAL4^dpp.blk1, Ser^UAS.cSa

(Lai et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Co-misexpression of mib1^{ΔRF.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} with Dl^Scer\UAS.cDa along the anteroposterior compartment boundary of the wing disc using Scer\GAL4^dpp.blk1 completely suppresses the ectopic margin or disc overgrowth seen with Dl^Scer\UAS.cDa expression alone. The expression of Dl^Scer\UAS.cDa is unable to rescue the loss of endogenous wing margin induced by mib1^{ΔRF.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC}. The wing phenotype caused by Ser^Scer\UAS.cSa under the control of Scer\GAL4^dpp.blk1 is completely suppressed by mib1^{ΔRF.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} coexpression. Expression of neur^Scer\UAS.cLa partially rescues the wing phenotype of flies that express mib1^{ΔRF.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} under the control of Scer\GAL4^dpp.blk1. This phenotype is almost completely suppressed when both mib1^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} and two copies of neur^Scer\UAS.cLa are expressed under the control of Scer\GAL4^dpp.blk1 alongside mib1^{ΔRF.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC}.

(Lai et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mib1^{ΔRF.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC}

mib1^{ΔRF.UAS.Tag:polyHis,Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close