FlyBase Allele Report: Dmel\cv-c[UAS.cDa]

General Information

Symbol

Dmel\cv-c^UAS.cDa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Denholm

FlyBase ID

FBal0191512

Feature type

allele

Associated gene

Dmel\cv-c

Associated Insertion(s)

Carried in Construct

P{UAS-cv-c.D}

Also Known As

UAS-Cv-c

Key Links

Transgenic product class

wild_type

(Denholm et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Denholm et al., 2005)

Mutagen

in vitro construct

(Denholm et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-cv-c.D}

Cytology

Description

UASt sequences drive expression of full-length cv-c.

(Denholm et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

cv-c

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology, with Scer\GAL4^RapGAP1-OK6, cv-c¹

(Pilgram et al., 2011)

Phenotype Manifest In

embryonic Malpighian tubule, with Scer\GAL4^ct.CtB

(Denholm et al., 2005)

embryonic Malpighian tubule tip cell, with Scer\GAL4^ct.CtB

(Denholm et al., 2005)

neuromuscular junction, with Scer\GAL4^RapGAP1-OK6, cv-c¹

(Pilgram et al., 2011)

posterior spiracle primordium, with Scer\GAL4^ems.HRE

(Simões et al., 2006)

Detailed Description

Statement

Reference

The expression of cv-c^Scer\UAS.cDa under the control of Scer\GAL4^GMR23E10 in cv-c^C524/cv-c^MB03717 heterozygous adults does not result in significant changes in the sensitivity to electric shocks, as assessed by maximal motor velocities, or in the preference score to the 4-methylcyclohexanol odorant in ad libitum sleep conditions, as compared to controls.

(Donlea et al., 2014)

Expression of cv-c^Scer\UAS.cDa in motoneurons under the control of Scer\GAL4^OK6 fails to rescue the abnormal quantal content (QC) phenotype of cv-c¹/+ larvae.

Expression of cv-c^Scer\UAS.cDa in muscle under the control of Scer\GAL4^G14 rescues the abnormal quantal content (QC) phenotype of cv-c¹/+ larvae.

(Pilgram et al., 2011)

Expression of cv-c^Scer\UAS.cDa under the control of Scer\GAL4^ems.HRE results in a failure of invagination of the most distal cells of the posterior spiracular chamber.

(Simões et al., 2006)

Malpighian tubules that express cv-c^Scer\UAS.cDa under the control of Scer\GAL4^ct.CtB are significantly shorter in length and fatter around their circumference than wild-type tubules. The length of the tip cell is increased; the tip cell stalk is ~7μ in wild-type tubules, but can be up to 50μ in Scer\GAL4^ct.CtB>cv-c^Scer\UAS.cDa tubules.

(Denholm et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Scer\GAL4^G14/cv-c^UAS.cDa is a non-suppressor of abnormal neurophysiology | dominant phenotype of Dys^E6

(Pilgram et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

cv-c^UAS.cDa/Scer\GAL4^69B is a suppressor of tracheal primordium phenotype of Egfr^f2

(Brodu and Casanova, 2006)

cv-c^UAS.cDa/Scer\GAL4^69B is a suppressor of presumptive embryonic/larval tracheal system phenotype of Egfr^f2

(Brodu and Casanova, 2006)

NOT Suppressor of

Statement

Reference

Scer\GAL4^G14/cv-c^UAS.cDa is a non-suppressor of neuromuscular junction phenotype of Dys^E6

(Pilgram et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The increased quantal content (QC) measured at the neuromuscular junction of Dys^E6/+ mutants is not reduced as a result of expression of cv-c^Scer\UAS.cDa under the control of Scer\GAL4^G14.

(Pilgram et al., 2011)

Expression of cv-c^Scer\UAS.cDa, under the control of Scer\GAL4^69B partially suppresses the Egfr^f2 mutant invagination phenotype.

(Brodu and Casanova, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^104y/cv-c^UAS.cDa rescues cv-c^C524/cv-c^MB03717

(Donlea et al., 2014)

Scer\GAL4^cv-c-C5/cv-c^UAS.cDa rescues cv-c^C5/cv-c^MB03717

(Donlea et al., 2014)

Scer\GAL4^G14/cv-c^UAS.cDa rescues cv-c¹

(Pilgram et al., 2011)

Partially rescues

Scer\GAL4^GMR23E10/cv-c^UAS.cDa partially rescues cv-c^C524/cv-c^MB03717

(Donlea et al., 2014)

Scer\GAL4^ct.CtB/cv-c^UAS.cDa partially rescues cv-c^M62

(Denholm et al., 2005)

Fails to rescue

Scer\GAL4^RapGAP1-OK6/cv-c^UAS.cDa fails to rescue cv-c¹

(Pilgram et al., 2011)

Comments

The expression of cv-c^Scer\UAS.cDa under the control of Scer\GAL4^cv-c-C5 (the intrinsic Gal4 driver of cv-c^C5) or of Scer\GAL4^104Y fully rescues the decreased mean sleep time exhibited by cv-c^C5/cv-c^MB03717 or cv-c^C524/cv-c^MB03717 transheterozygous adults, respectively, reared under 12h light:12h dark cycles.

The expression of cv-c^Scer\UAS.cDa under the control of Scer\GAL4^GMR23E10 rescues the decreased mean sleep time, the lower sleep recovery after overnight sleep deprivation and the short-term olfactory memory decrease under ad libitum sleep conditions exhibited by cv-c^C524/cv-c^MB03717; expression in these mutants does not significantly alter the sensitivity to electric shocks, as assessed by maximal motor velocities, nor the preference score to the 4-methylcyclohexanol odorant in ad libitum sleep conditions.

(Donlea et al., 2014)

Expression of cv-c^Scer\UAS.cDa in motoneurons under the control of Scer\GAL4^OK6 fails to rescue the abnormal quantal content (QC) phenotype of cv-c¹/+ larvae.

Expression of cv-c^Scer\UAS.cDa in muscle under the control of Scer\GAL4^G14 rescues the abnormal quantal content (QC) phenotype of cv-c¹/+ larvae.

(Pilgram et al., 2011)

Expression of cv-c^Scer\UAS.cDa, under the control of Scer\GAL4^ct.CtB, partially rescues the Malpighian tubule phenotype of cv-c^M62 embryos.

(Denholm et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

cv-c^Scer\UAS.cDa

cv-c^UAS.cDa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Denholm

(Denholm et al., 2005)

Secondary FlyBase IDs

References (7)

Report Sections

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