FB2026_02 , released June 18, 2026
Allele: Dmel\Prat2RNAi.AS.UAS
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General Information
Symbol
Dmel\Prat2RNAi.AS.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0193127
Feature type
allele
Associated gene
Dmel\Prat2
Associated Insertion(s)
Carried in Construct
P{UAS-Prat2.RNAi.AS}
Key Links
Transgenic product class
RNAi_reagent
(Ji and Clark, 2006)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Ji and Clark, 2006)
Mutagen
in vitro construct
(Ji and Clark, 2006)
Progenitor genotype
Carried in construct
P{UAS-Prat2.RNAi.AS}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat of a 216bp Prat2 fragment (166bp of 5' UTR sequence and 50bp of the propeptide sequence) in an antisense-sense orientation, separated by a 95bp spacer.

(Ji and Clark, 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Prat2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Prat2dsRNA.IR-AS.Scer\UAS under the control Scer\GAL4Act5C.PI results in varying degrees of lethality, depending on the P{UAS-Prat.IR-AS} line used, the copy number and the temperature at which the flies are raised.

      Expression of Prat2dsRNA.IR-AS.Scer\UAS under the control Scer\GAL4αTub84B.PL results in 50% of arrested pupae having a "cryptocephal" phenotype, consisting of head eversion defects, legs that are not fully elongated, an abdomen that lacks cuticle and segmentation and larval mouthparts still being attached to the imaginal head structure. This cryptocephal phenotype is also seen in animals expressing Prat2dsRNA.IR-AS.Scer\UAS under the control Scer\GAL4Act5C.PI.

      Rarely, animals expressing Prat2dsRNA.IR-AS.Scer\UAS under the control Scer\GAL4Act5C.PI have a "microcephal" phenotype, which results from partial head eversion. These pupae retain their larval mouthparts and do not have a fully differentiated abdomen.

      Surviving female adults expressing Prat2dsRNA.IR-AS.Scer\UAS under the control Scer\GAL4αTub84B.PL are fertile.

      (Ji and Clark, 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of both Prat2dsRNA.IR-AS.Scer\UAS and PratdsRNA.IR-AS.Scer\UAS under the control of Scer\GAL4αTub84B.PL results in a higher degree of female lethality than that seen when either Prat2dsRNA.IR-AS.Scer\UAS or PratdsRNA.IR-AS.Scer\UAS is expressed alone under the control of Scer\GAL4αTub84B.PL. 27% of the double mutant animals arrest at a very early prepupal stage. Some of these arrested prepupae retain the thin and long shape typical of larvae, the anterior spiracle fails to evert and the gas bubble remains in the middle of the body. 34% of the double mutant animals arrest at prepupal stage P4(i) and have head eversion defects and a shrunken abdomen, and the gas bubble is in the posterior of the animal. 24% of the double mutant animals show head eversion, but the head is partially collapsed, resulting in a large cavity in the anterior of the pupa. 7% of the double mutant animals have a "cryptocephal" phenotype and 5% die as pharate adults.

      (Ji and Clark, 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Used to study the phenotypic consequences of dsRNA interference (RNAi) of the Prat2 gene.

      (Ji and Clark, 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Prat2RNAi.AS.UAS
      Prat2RNAi.IR-AS.UAS
      Prat2dsRNA.IR-AS.Scer\UAS
      Prat2dsRNA.IR-AS.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)