FlyBase Allele Report: Dmel\nkd::Mmus\Nkd1[f30aa.UAS.EGFP]

General Information

Symbol

Dmel\nkd::Mmus\Nkd1^{f30aa.UAS.EGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0193323

Feature type

allele

Associated gene

nkd::Mmus\Nkd1

Associated Insertion(s)

Carried in Construct

P{UAS-nkd::Mmus\Nkd1.f30aa.GFPC}

Also Known As

mNkd1^f30aa/GFPC

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Waldrop et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-nkd::Mmus\Nkd1.f30aa.GFPC}

Cytology

Description

UASt regulatory sequences drive expression of Mmus\Nkd1 in which amino acid residues 248-274 have been replaced with amino acid residues 543-572 of nkd. The fusion protein is tagged at the C-terminal end with EGFP.

(Waldrop et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

nkd::Mmus\Nkd1

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scer\GAL4^B119, nkd::Mmus\Nkd1^{f30aa.UAS.EGFP}, wg^l-12/wg[+] has visible phenotype

(Waldrop et al., 2006)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.G32/nkd::Mmus\Nkd1^{f30aa.UAS.EGFP} is a non-suppressor of embryonic/first instar larval cuticle phenotype of nkd³

(Chan et al., 2007)

Scer\GAL4^da.G32/nkd::Mmus\Nkd1^{f30aa.UAS.EGFP} is a non-suppressor of ventral denticle belt phenotype of nkd³

(Chan et al., 2007)

Scer\GAL4^da.G32/nkd::Mmus\Nkd1^{f30aa.UAS.EGFP} is a non-suppressor of embryonic/first instar larval cuticle phenotype of nkd³/nkd²

(Waldrop et al., 2006)

Other

Statement

Reference

Scer\GAL4^B119, nkd::Mmus\Nkd1^{f30aa.UAS.EGFP}, wg^l-12/wg[+] has abdominal sternite phenotype

(Waldrop et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of nkd::Mmus\Nkd1^{f30aa.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 does not rescue the nkd³ cuticle phenotype.

(Chan et al., 2007)

Expression of nkd::Mmus\Nkd1^{f30aa.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 fails to rescue the cuticle defects of nkd²/nkd³ embryos.

Expression of nkd::Mmus\Nkd1^{f30aa.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^B119 in a wg^l-12/+ background results in a reduction in the number of sternite bristles on the abdomen.

(Waldrop et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mNkd1^f30aa/GFPC

(Chan et al., 2007, Waldrop et al., 2006)

nkd::Mmus\Nkd1^{f30aa.Scer\UAS.T:Avic\GFP-EGFP}

nkd::Mmus\Nkd1^{f30aa.UAS.EGFP}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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