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General Information
Symbol
Dmel\nkd3
Species
D. melanogaster
Name
FlyBase ID
FBal0013025
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
nkd7H16
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C19010945T

Amino acid change:

Q60term | nkd-PA; Q60term | nkd-PB; Q60term | nkd-PC

Reported amino acid change:

Q60term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q60term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mutant embryos secrete a uniform naked cuticle.

nkd2/nkd3 embryos have a strong cuticle phenotype with a severe head involution defect and widely split posterior spiracles.

nkd2/nkd3 embryos have a strong cuticle phenotype; the cuticles are less than 75% of wild-type length, have two or less complete denticle bands and have an exteriorized head skeleton and noneverted spiracles.

Homozygous mutant nkd3 eyes, generated by somatic clone, are small and rough. Homozygous embryos lack denticle belts.

Homozygous embryos have a strong cuticle phenotype; they secrete cuticle with a fully exteriorised head skeleton, widely split filzkorper and residual denticle belts only in segment A3 and/or A5 (or no denticle belts at all), and are typically less than 75% of wild-type length.

ptc parasegmental boundaries are shifted in nkd embryos forming posterior to each en domain: expression domain is reduced.

nkd mutant embryos have Keilin's organs, but these often have four or more sensory hairs rather than the normal three. Occasionally extra, discrete organs are seen, so that three organs appear in one segment, usually accompanied by the loss of an organ from an adjacent segment. nkd3/nkd2 embryos grew in in vivo culture, producing implants containing imaginal discs and all larval tissues. The discs tended to grow as a merged sheet of material. Metamorphosed implants produced adult cuticular structures derived from the eye-antenna discs, leg and wing discs.

strong allele

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement
Reference
Phenotype Manifest In
Suppressed by
NOT suppressed by
NOT Enhancer of
Statement
Reference

nkd3 is a non-enhancer of phenotype of dsh3

nkd3 is a non-enhancer of phenotype of arm4

NOT Suppressor of
Statement
Reference

nkd3 is a non-suppressor of phenotype of dsh3

nkd3 is a non-suppressor of phenotype of arm4

Other
Additional Comments
Genetic Interactions
Statement
Reference

h1 nkd3 double homozygous, h1/h1 nkd3/nkd13 double mutant or h1/h1 nkd2/nkd3 double mutant embryos exhibit defective denticle belts.

The naked cuticle phenotype of nkd3 embryos is partially rescued by expression of panΔN.Scer\UAS under the control of either Scer\GAL4ptc-559.1 or Scer\GAL4en-e16E, with more complete rescue being seen when Scer\GAL4ptc-559.1 is used as the driver.

nkd3 h1 clones give rise to a rough eye phenotype and loss of wing margin bristles.

Xenogenetic Interactions
Statement
Reference

Expression of nkd::Mmus\Nkd1m1NΔN.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4da.G32 results in little or no rescue of the nkd3 cuticle phenotype.

Expression of nkd::Mmus\Nkd1f30aa.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 does not rescue the nkd3 cuticle phenotype.

Expression of Mmus\Nkd1Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 does not rescue the nkd3 cuticle phenotype.

Expression of nkd::Mmus\Nkd1fNf30aa.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 partially rescues the nkd3 cuticle phenotype.

Expression of nkd::Btau\recoverinrEF.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.G32 largely rescues the cuticle defects of nkd2/nkd3 embryos and adult viability is restored.

Expression of nkd::Hsap\NKD2hEFX2.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.G32 largely rescues the cuticle defects of nkd2/nkd3 embryos and adult viability is restored.

Expression of Mmus\Nkd1Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 fails to rescue the cuticle defects of nkd2/nkd3 embryos.

Expression of nkd::Mmus\Nkd1f30aa.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 fails to rescue the cuticle defects of nkd2/nkd3 embryos.

When Xlae\Gsk3βcRa RNA is injected into nkd3 flies a suppression of the naked cuticle phenotype is seen leading to the production of some denticle belts.

Complementation and Rescue Data
Fails to complement
Partially rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (4)
Notes on Origin
Discoverer
Comments
Comments

The expression of l(1)sc is unaltered in early homozygous mutant embryos.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (18)