FlyBase Allele Report: Dmel\nkd[NBam.UAS.EGFP]

General Information

Symbol

Dmel\nkd^{NBam.UAS.EGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0146978

Feature type

allele

Associated gene

Dmel\nkd

Associated Insertion(s)

Carried in Construct

P{UAS-nkd.NBam.GFPC}

Key Links

Transgenic product class

inframe_deletion

(Rousset et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Rousset et al., 2002)

Mutagen

in vitro construct

(Rousset et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-nkd.NBam.GFPC}

Cytology

Description

UASt regulatory sequences drive expression of nkd which lacks amino acid residues 296-928 and is tagged at the C-terminal end with EGFP.

(Waldrop et al., 2006)

UAS regulatory sequences drive expression of amino acids 1 to 294 of nkd, tagged with EGFP at the C-terminal end.

(Rousset et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

UASt

binary expression system - regulatory region

Encoded product / tool

nkd

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of P-stage, with Scer\GAL4^A8

(Rousset et al., 2002)

some die during pharate adult stage, with Scer\GAL4^A8

(Rousset et al., 2002)

visible, with Scer\GAL4^B119

(Rousset et al., 2002)

Phenotype Manifest In

abdominal sternite bristle, with Scer\GAL4^A8

(Rousset et al., 2002)

abdominal sternite bristle, with Scer\GAL4^B119

(Rousset et al., 2002)

leg, with Scer\GAL4^A8

(Rousset et al., 2002)

wing, with Scer\GAL4^A8

(Rousset et al., 2002)

Detailed Description

Statement

Reference

Expression of nkd^{NBam.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^B119 results in flies with largely normal sternite bristle patterns and flies with subtle sternite bristle pattern abnormalities, most commonly loss of hemisternite bristle clusters. Expression of nkd^{NBam.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^A8 results in pharate adults which fail to hatch, with small wings, short legs and sternite bristle abnormalities.

(Rousset et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

nkd^{NBam.UAS.EGFP}/Scer\GAL4^da.G32 fails to rescue nkd³/nkd²

(Waldrop et al., 2006)

nkd^{NBam.UAS.EGFP}/Scer\GAL4^da.G32 fails to rescue nkd³/nkd²

(Rousset et al., 2002)

Comments

Expression of nkd^{NBam.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 fails to rescue the cuticle defects of nkd²/nkd³ embryos.

(Waldrop et al., 2006)

Does not rescue the cuticle defects of nkd²/nkd³ embryos when expressed under the control of Scer\GAL4^da.G32.

(Rousset et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Nkd^NBam/GFPC

(Waldrop et al., 2006)

nkd^{NBam.Scer\UAS.T:Avic\GFP-EGFP}

nkd^{NBam.Scer\UAS.T:Avic\GFP}

nkd^{NBam.UAS.EGFP}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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