FlyBase Allele Report: Dmel\ds[ΔECD.UAS]

General Information

Symbol

Dmel\ds^ΔECD.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0193593

Feature type

allele

Associated gene

Dmel\ds

Associated Insertion(s)

Carried in Construct

P{UAS-ds.ΔECD}

Also Known As

UAS-dsΔECD, DsΔECD

Key Links

Transgenic product class

inframe_deletion

(Matakatsu and Blair, 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Matakatsu and Blair, 2006)

Mutagen

in vitro construct

(Matakatsu and Blair, 2006)

Progenitor genotype

ds^UAS.cTa

(Matakatsu and Blair, 2006)

Carried in construct

P{UAS-ds.ΔECD}

Cytology

Description

Internal deletion of the ds coding region from R143 to G3080 that removes the cadherin repeats and ends 17 amino acids from the predicted transmembrane domain.

(Matakatsu and Blair, 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number, with Scer\GAL4^hh.PU, ds^UAO71

(Degoutin et al., 2013)

lethal, with Scer\GAL4^αTub84B.PL, ds⁰⁵¹⁴²

(Matakatsu and Blair, 2006)

visible, with Scer\GAL4^hh-Gal4

(Matakatsu and Blair, 2012)

Phenotype Manifest In

crossvein, with Scer\GAL4^en-e16E

(Matakatsu and Blair, 2006)

wing, with Scer\GAL4^en-e16E

(Matakatsu and Blair, 2006)

wing, with Scer\GAL4^hh-Gal4

(Matakatsu and Blair, 2012)

wing, with Scer\GAL4^αTub84B.PL

(Matakatsu and Blair, 2006)

wing disc, with Scer\GAL4^hh.PU, ds^UAO71

(Degoutin et al., 2013)

wing disc posterior compartment, with Scer\GAL4^hh-Gal4

(Matakatsu and Blair, 2012)

Detailed Description

Statement

Reference

Expression of ds^{ΔECD.Scer\UAS} under the control of Scer\GAL4^hh.PU in a ds^UAO71 mutant background produces wing disc tissue overgrowth, particularly in the hinge region.

(Degoutin et al., 2013)

Expression of ds^{ΔECD.Scer\UAS} under the control of Scer\GAL4^hh-Gal4 induces overgrowth of the posterior compartment in the wing disc and adult wing.

(Matakatsu and Blair, 2012)

Expression of ds^{ΔECD.Scer\UAS} in the wing does not affect planar cell polarity when driven by Scer\GAL4^salm-459.2, Scer\GAL4^Dll-md23, Scer\GAL4^ap-md544, Scer\GAL4^αTub84B.PL or Scer\GAL4^en-e16E.

Wings that express ds^{ΔICD.Scer\UAS} under the control of Scer\GAL4^en-e16E causes a foreshortening along the proximodistal axis of the wing blade as the anterior and posterior crossveins are abnormally close and crossveins are partially missing. A mild version of this defect is observed when ds^{ΔECD.Scer\UAS} is driven by Scer\GAL4^αTub84B.PL.

(Matakatsu and Blair, 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^hh.PU, ds^ΔECD.UAS, ds^UAO71 has increased cell number phenotype, suppressible | partially by wap^RNAi.UAS.cUa/Scer\GAL4^hh.PU

(Degoutin et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^hh.PU, ds^ΔECD.UAS, ds^UAO71 has wing disc phenotype, suppressible | partially by wap^RNAi.UAS.cUa/Scer\GAL4^hh.PU

(Degoutin et al., 2013)

Other

Statement

Reference

Scer\GAL4^Act5C.PI, ds^ΔECD.UAS, ft^ΔECD.UAS has S2 cell-line phenotype

(Matakatsu and Blair, 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of wap^VDRC.cUa substantially suppresses the wing disc morphological defects seen when ds^{ΔECD.Scer\UAS} is expressed under the control of Scer\GAL4^hh.PU in a ds^UAO71 mutant background.

(Degoutin et al., 2013)

S2 cells that express with ds^{ΔECD.Scer\UAS} and ft^{ΔECD.Scer\UAS}, under the control of Scer\GAL4^Act5C.PI, fail to aggregate.

(Matakatsu and Blair, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

ds^ΔECD.UAS/Scer\GAL4^ds-gal4 fails to rescue ds⁰⁵¹⁴²

(Matakatsu and Blair, 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into S2 cells for use in cell aggregation assays.

(Matakatsu and Blair, 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ds^{ΔECD.Scer\UAS}

ds^ΔECD.UAS

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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