FlyBase Allele Report: Dmel\Calx[ninaE.PW]

General Information

Symbol

Dmel\Calx^ninaE.PW

Species

D. melanogaster

Name

neither inactivation nor afterpotential E promoter construct of Wang

FlyBase ID

FBal0193743

Feature type

allele

Associated gene

Dmel\Calx

Associated Insertion(s)

Carried in Construct

P{ninaE-Calx.W}

Also Known As

ninaE-calx

Key Links

Transgenic product class

cDNA

(Wang et al., 2005)

wild_type

(Wang et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Wang et al., 2005)

wild_type

(Wang et al., 2005)

Mutagen

in vitro construct

(Wang et al., 2005)

Progenitor genotype

Carried in construct

P{ninaE-Calx.W}

Cytology

Description

A ninaE promoter drives expression of Calx1 cDNA (sequence corresponds to AT10348).

(Wang et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

ninaE

Encoded product / tool

Calx

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates combined saposin deficiency

modeled by Sap-r^e01294

(Hindle et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology, with Calx^A

(Wang et al., 2005)

Phenotype Manifest In

eye, with Calx^A

(Wang et al., 2005)

Detailed Description

Statement

Reference

Expression of Calx^ninaE.PW does not result in significant degeneration of visual function in the eye as measured via electroretinogram recording in 5 day-old versus 22 day-old adults.

(Hindle et al., 2017)

The amplitude and integral currents (called quantum bumps) generated by a single photon of light is much smaller in Calx^A flies compared to wild-type flies. The small quantum bump amplitudes and waveforms produced by these mutants correlate with low Na/Ca currents. In contrast, Calx^A flies that express Calx^ninaE.PW generate quantum bumps that are larger than wild-type and correlate with high Na/Ca currents.

(Wang et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Calx^ninaE.PW is an enhancer of abnormal neurophysiology | adult stage | progressive phenotype of Df(3R)tll-e/Sap-r^C27

(Hindle et al., 2017)

Suppressor of

Statement

Reference

Calx^ninaE.PW is a suppressor | partially of abnormal neuroanatomy | adult stage | progressive phenotype of Cpn^RNAi.UAS.WIZ, Scer\GAL4^GMR.PU

(Weiss et al., 2012)

Calx^ninaE.PW is a suppressor | partially of abnormal neurophysiology | adult stage phenotype of Cpn^RNAi.UAS.WIZ, Scer\GAL4^GMR.PU

(Weiss et al., 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

Calx^ninaE.PW is an enhancer of eye | progressive phenotype of Df(3R)tll-e/Sap-r^C27

(Hindle et al., 2017)

Suppressor of

Statement

Reference

Calx^ninaE.PW is a suppressor | partially of rhabdomere phenotype of Cpn^RNAi.UAS.WIZ, Scer\GAL4^GMR.PU

(Weiss et al., 2012)

Calx^ninaE.PW is a suppressor of retina phenotype of trp^P365

(Wang et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Calx^ninaE.PW exacerbates the age-related deterioration of visual function seen in Sap-r^C27/Df(3R)tll-e mutants, as measured via electroretinogram recording.

(Hindle et al., 2017)

Calx^ninaE.PW significantly rescues the degenerated rhabdomere morphology seen in flies which have been exposed to light and which are expressing Cpn^{dsRNA.Scer\UAS.WIZ} under the control of a Scer\GAL4^GMR.PU line which results in Cpn expression being reduced to only 1% of wild-type levels. In addition, the reduction in electroretinogram response amplitude is partially rescued at moderate and intense light levels. The normal electron transparent appearance of the rhabdomere terminal web is not rescued.

(Weiss et al., 2012)

The retinal degeneration seen in trp^P365/+ flies reared in the dark for 10 days is almost completely suppressed by expression of Calx^ninaE.PW.

(Wang et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Calx^ninaE.PW partially rescues Calx^A

(Wang et al., 2005)

Comments

Expression of Calx^ninaE.PW in Calx^A photoreceptor cells rescues the ability of the Calx^A photoreceptors to produce a Na⁺/Ca⁺ exchange current; in fact, this exchange current is ~8-fold larger and ~5-fold faster than that produced by wild-type cells. Additionally, expression of Calx^ninaE.PW prevents the age-dependent loss of the deep pseudopupil from the eyes of Calx^A flies. Calx^ninaE.PW; Calx^A eyes have rhabdomeres 1-6, which are rarely present in Calx^A eyes (rhabdomere 7 is not rescued by Calx^ninaE.PW due to its expression pattern).

(Wang et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Calx^ninaE.PW

Name Synonyms

neither inactivation nor afterpotential E promoter construct of Wang

Secondary FlyBase IDs

References (7)

Report Sections

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