FlyBase Allele Report: Dmel\ave[108V]

General Information

Symbol

Dmel\ave^108V

Species

D. melanogaster

Name

FlyBase ID

FBal0193994

Feature type

allele

Associated gene

Dmel\ave

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Roignant et al., 2006)

Mutagen

ethyl methanesulfonate

(Roignant et al., 2006)

Progenitor genotype

Cytology

Description

Amino acid replacement: W34term.

(Roignant et al., 2006)

The stop mutation is within the 'SAM' domain.

(Roignant et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:14,752,690..14,752,690 [+]

Nucleotide change:

G14752690A

Amino acid change:

W34term | ave-PA

Reported amino acid change:

W34term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Roignant et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | somatic clone

(Roignant et al., 2006)

increased cell death | somatic clone

(Roignant et al., 2006)

lethal - all die before end of larval stage | recessive

(Roignant et al., 2006)

some die during third instar larval stage | recessive

(Roignant et al., 2006)

Phenotype Manifest In

Detailed Description

Statement

Reference

ave^108V mutant clones generated in third instar eye discs lack R1 and R6 photoreceptors, have a reduced number of R3 and R4 cells and have very few cone cells. ave^108V expression in these clones indicates a partial failure of G1 arrest and there appears to be a higher level of cell death within the clones.

The ave^108V mutation affects wing development. Induction of ave^108V mutant clones results in a loss of wing veins in the adult wing. Furthermore, the wing discs of homozygous ave^108V third instar larvae show greatly reduced notum primordia.

(Roignant et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

ave^108V has photoreceptor cell R1 | somatic clone phenotype, suppressible by Scer\GAL4^da.G32/Raf^UAS.F179

(Roignant et al., 2006)

ave^108V has photoreceptor cell R6 | somatic clone phenotype, suppressible by Scer\GAL4^da.G32/Raf^UAS.F179

(Roignant et al., 2006)

ave^108V has photoreceptor cell R4 | somatic clone phenotype, suppressible by Scer\GAL4^da.G32/Raf^UAS.F179

(Roignant et al., 2006)

ave^108V has photoreceptor cell R3 | somatic clone phenotype, suppressible by Scer\GAL4^da.G32/Raf^UAS.F179

(Roignant et al., 2006)

NOT suppressed by

Statement

Reference

ave^108V has photoreceptor cell R1 | somatic clone phenotype, non-suppressible by Ras85D^V12.UAS/Scer\GAL4^da.G32

(Roignant et al., 2006)

ave^108V has photoreceptor cell R6 | somatic clone phenotype, non-suppressible by Ras85D^V12.UAS/Scer\GAL4^da.G32

(Roignant et al., 2006)

ave^108V has photoreceptor cell R4 | somatic clone phenotype, non-suppressible by Ras85D^V12.UAS/Scer\GAL4^da.G32

(Roignant et al., 2006)

ave^108V has photoreceptor cell R3 | somatic clone phenotype, non-suppressible by Ras85D^V12.UAS/Scer\GAL4^da.G32

(Roignant et al., 2006)

Suppressor of

Statement

Reference

ave^108V is a suppressor of eye photoreceptor cell | ectopic phenotype of Ras85D^V12.UAS, Scer\GAL4^da.G32

(Roignant et al., 2006)

NOT Suppressor of

Statement

Reference

ave^108V is a non-suppressor of eye photoreceptor cell | ectopic phenotype of Raf^UAS.F179, Scer\GAL4^da.G32

(Roignant et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Mutant clones that express Ras85D^V12.Scer\UAS, under the control of Scer\GAL4^da.G32, in a ave^108V background show the ave^108V photoreceptor differentiation phenotype. The Scer\GAL4^da.G32>Ras85D^V12.Scer\UAS ectopic photoreceptor phenotype is therefore suppressed. In contrast, when phl^{Scer\UAS.F179} is expressed under the control of Scer\GAL4^da.G32 in ave^108V mutant clones, ectopic photoreceptor cells are induced and the ave^108V phenotype is suppressed.

(Roignant et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

ave^108V is partially rescued by Scer\GAL4^da.G32/ave^UAS.Tag:HA

(Roignant et al., 2006)

Comments

Neither ave^CC9-36B nor ave^CC9-20A can complement the lethality ave^108V.

(Wolfstetter et al., 2017)

Expression of ave^{Scer\UAS.T:Ivir\HA1}, under the control of Scer\GAL4^da.G32, in ave^108V mutant clones of the eye disc rescues the photoreceptor differentiation defect.

(Roignant et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ave^108V

(Wolfstetter et al., 2017, Roignant et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close