1 day memory is impaired after spaced training, but not after massed training in an olfactory associative conditioning assay in flies expressing Tequila^{dsRNA.Scer\UAS} under the control of Scer\GAL4^G0431. The defect in memory after spaced training is still seen if the temperature sensitive Scer\GAL80^ts.αTub84B allele is used to restrict Scer\GAL4 expression to the adult stage.

Expression of Tequila^{dsRNA.Scer\UAS} in the adult mushroom bodies, under the control of Scer\GAL4^{Mef2.247.Switch} induces defects in both sugar response and short-term memory.

RNAi-mediated Tequila knockdown, achieved by expression of Tequila^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mef2.247, results in a decrease in 24-hour long-term memory. 24-hour memory after massed training is unaffected , as are learning and 2-hour memory after single conditioning. No alteration of mushroom body morphology can be observed. The long-term memory phenotype induced by RNAi knockdown is reversible as flies expressing Tequila^{dsRNA.Scer\UAS} driven by Scer\GAL4^{Mef2.247.Switch} show the phenotype while flies are fed with RU486, but show a normal 24-hour long term memory capacity following transferral to food without RU846, thus preventing Scer\GAL4^{Mef2.247.Switch} from driving Tequila^{dsRNA.Scer\UAS} expression.

RNAi-mediated Tequila knockdown, achieved by expression of Tequila^{dsRNA.Scer\UAS} under the control of either Scer\GAL4⁵¹²² or Scer\GAL4^238Y, results in a decrease in 24-hour long-term memory. No alteration of mushroom body morphology is observed in these flies.