Amino acid replacement: S236A.
Amino acid replacement: S237A.
The endogenous dsh promoter (2.9kb of genomic sequences from upstream of the dsh gene) drives expression of dsh, in which all five of the conserved S/T residues have been mutated. The dsh sequence is tagged at the 3' end with EGFP.
Amino acid replacement: S239A.
Amino acid replacement: S240A.
Amino acid replacement: T242A.
dshST5.EGFP rescues dsh1
dshST5.EGFP rescues dsh3