FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\SerY1248S.F2I.UAS
Open Close
General Information
Symbol
Dmel\SerY1248S.F2I.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0194254
Feature type
allele
Associated gene
Dmel\Ser
Associated Insertion(s)
Carried in Construct
P{UAS-Ser.EI}
Key Links
Transgenic product class
missense_variant
(Li and Baker, 2004)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Li and Baker, 2004)
Mutagen
in vitro construct
(Li and Baker, 2004)
Progenitor genotype
Carried in construct
P{UAS-Ser.EI}
Cytology
Description

Amino acid replacement: Y1248S.

(Li and Baker, 2004)

UASt regulatory sequences drive expression of an engineered Ser cDNA. Nucleotides encoding Tyr1248 and Arg1249 are replaced with an XbaI restriction site (which encodes Ser and Arg), and the initiating ATG codon is replaced with a BspH1 restriction site which results in replacing Phe2 with Ile. The endogenous Ser 5'UTR is replaced with a HindIII-NcoI fragment of the Xenopus β-globin gene leader. The 3'UTR of the Ser cDNA is truncated to 17bp 3' of the stop codon and SV40 polyadenylation sequences are present.

(Li and Baker, 2004)

Amino acid replacement: F2I.

(Li and Baker, 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Ser
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      eye photoreceptor cell & eye disc, with Scer\GAL4h-H10

      (Li and Baker, 2004)

      eye photoreceptor cell & eye disc | posterior, with Scer\GAL4sca-537.4

      (Li and Baker, 2004)

      macrochaeta & scutellum | supernumerary | somatic clone | cell autonomous, with Scer\GAL4sca-109-68

      (Li and Baker, 2004)

      macrochaeta & thorax | somatic clone, with Scer\GAL4Ubx.PdC

      (Li and Baker, 2004)

      macrochaeta & thorax | supernumerary, with Scer\GAL4sca-537.4

      (Li and Baker, 2004)

      macrochaeta & thorax | supernumerary | somatic clone, with Scer\GAL4Ubx.PdC

      (Li and Baker, 2004)
      Detailed Description
      Statement
      Reference

      Expression of SerY1248S.F2I.Scer\UAS under the control of Scer\GAL4sca-537.4 causes a reduction in the number of photoreceptors R1-7 posterior to the morphogenetic furrow of developing eyes, and an extreme neurogenic phenotype on the adult thorax, converting all proneural cells into bristle precursors.

      Expression of SerY1248S.F2I.Scer\UAS under the control of Scer\GAL4h-H10 is strongly neurogenic, resulting in a large excess of R8-like cells as well as an increase in other photoreceptor cell types in the eye imaginal disc.

      Clonal expression of SerY1248S.F2I.Scer\UAS in thoracic proneural regions (using Scer\GAL4sca-109-68) leads to extra scutellar bristle differentiation. In nearly all cases where mosaic patches of bristles form, only one of the bristles derived from a non-SerY1248S.F2I.Scer\UAS-expressing cell, suggesting cell autonomous effects.

      Clonal expression of SerY1248S.F2I.Scer\UAS in thoracic cells (using Scer\GAL4Ubx.PdC) causes many extra thoracic bristles to differentiate. The morphology of these bristles is abnormal.

      (Li and Baker, 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Statement
      Reference

      SerY1248S.F2I.UAS/Scer\GAL4h-H10 is a non-suppressor of abnormal neuroanatomy | heat sensitive phenotype of Nint.hs

      (Li and Baker, 2004)
      Phenotype Manifest In
      NOT Suppressor of
      Statement
      Reference

      SerY1248S.F2I.UAS/Scer\GAL4h-H10 is a non-suppressor of eye photoreceptor cell & eye disc | conditional ts phenotype of Nint.hs

      (Li and Baker, 2004)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of SerY1248S.F2I.Scer\UAS under the control of Scer\GAL4h-H10 does not prevent the Nint.hs-induced inhibition of photoreceptor differentiation in a band of cells in the developing eye disc.

      (Li and Baker, 2004)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      SerY1248S.F2I.Scer\UAS
      SerY1248S.F2I.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)