FlyBase Allele Report: Dmel\capt[K304]

General Information

Symbol

Dmel\capt^K304

Species

D. melanogaster

Name

FlyBase ID

FBal0194511

Feature type

allele

Associated gene

Dmel\capt

Associated Insertion(s)

Carried in Construct

Also Known As

punc

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Medina et al., 2006)

Progenitor genotype

Cytology

Description

Amino acid replacement: K304term.

(Medina et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:1,152,458..1,152,458 [-]

Nucleotide change:

A1152458T

Amino acid change:

K304term | capt-PA; K663term | capt-PB; K304term | capt-PC

Reported amino acid change:

K304term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Medina et al., 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage

(Medina et al., 2008, Medina et al., 2006)

abnormal neuroanatomy | larval stage (with capt^E636)

(Medina et al., 2008)

abnormal neuroanatomy | larval stage (with Df(2L)ast5)

(Medina et al., 2008)

lethal (with capt^E636)

(Medina et al., 2008)

lethal (with Df(2L)ast5)

(Medina et al., 2008)

lethal (with Df(2L)Exel6004)

(Medina et al., 2008)

lethal (with Df(2L)Exel7006)

(Medina et al., 2008)

lethal | recessive

(Medina et al., 2006)

Phenotype Manifest In

dendrite | larval stage

(Medina et al., 2008, Medina et al., 2006)

dendrite | larval stage (with capt^E636)

(Medina et al., 2008)

dendrite | larval stage (with Df(2L)ast5)

(Medina et al., 2008)

Detailed Description

Statement

Reference

Homozygous, capt^K304/capt^E636 and capt^K304/Df(2L)ast5 larvae have punctate-like accumulations expressing Act5C^{Scer\UAS.T:Avic\GFP} in the dendrites of the sensory neuron dorsal cluster (these actin accumulations are absent in the dendrites of wild-type larvae).

The dynamics of the Act5C^{Scer\UAS.T:Avic\GFP} puncta phenotype in dendrites of capt^K304 larvae is dynamic, with accumulations dissipating within dendrites, while simultaneously new accumulations can form within a different part of the same dendrite.

Homozygotes have significantly fewer mitochondria in the dendrites than controls. The distribution of mitochondria within the cell bodies and in the axons are comparable to those of controls.

(Medina et al., 2008)

Homozygotes show defects in larval dendrite development; mutants have unusual actin patches (assayed using Act5C^{Scer\UAS.T:Avic\GFP} expression) in the dendrites and occasionally have neuritic outgrowths containing actin.

(Medina et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

capt^K304 has abnormal neuroanatomy phenotype, enhanceable by Khc^k13314

(Medina et al., 2008)

Enhancer of

Statement

Reference

punc[+]/capt^K304 is an enhancer of abnormal neuroanatomy phenotype of Khc^k13314

(Medina et al., 2008)

capt^K304/capt^K304 is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^109(2)80, tsr^UAS.cNa

(Medina et al., 2008)

Other

Statement

Reference

Khc^k13314, punc[+]/capt^K304 has abnormal neuroanatomy phenotype

(Medina et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

capt^K304 has dendrite phenotype, enhanceable by Khc^k13314

(Medina et al., 2008)

Enhancer of

Statement

Reference

capt^K304 is an enhancer of dendrite phenotype of Khc^k13314

(Medina et al., 2008)

capt^K304/capt^K304 is an enhancer of dendrite phenotype of Scer\GAL4^109(2)80, tsr^UAS.cNa

(Medina et al., 2008)

Other

Statement

Reference

Khc^k13314, capt^K304 has dendrite phenotype

(Medina et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Longer rod-like structures of Act5C^{Scer\UAS.T:Avic\GFP} accumulation are formed in the dendrites of animals expressing tsr^Scer\UAS.cNa under the control of Scer\GAL4^109(2)80 when the animals are also homozygous for capt^K304.

capt^K304 Khc^k13314 double homozygotes show a higher frequency of Act5C^{Scer\UAS.T:Avic\GFP} puncta in the dendrites and dendritic swellings than seen in either the capt^K304 or Khc^k13314 single homozygotes respectively. In addition, the double homozygotes show formation of elongate actin rods (expressing Act5C^{Scer\UAS.T:Avic\GFP}), a phenotype that is not seen in either single homozygote.

(Medina et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

capt^E636

(Medina et al., 2008)

Rescued by

capt^K304 is rescued by Scer\GAL4^109(2)80/capt^UAS.mCherry

(Medina et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

capt^K304

(Medina et al., 2008)

punc

(Medina et al., 2008, Medina et al., 2006)

punc¹

Name Synonyms

punctate

(Medina et al., 2008)

Secondary FlyBase IDs

References (2)

Report Sections

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