FlyBase Allele Report: Dmel\Pp2B-14D[act.UASp]

General Information

Symbol

Dmel\Pp2B-14D^act.UASp

Species

D. melanogaster

Name

FlyBase ID

FBal0194822

Feature type

allele

Associated gene

Dmel\Pp2B-14D

Associated Insertion(s)

Carried in Construct

P{UASp-Pp2B-14D.act}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Takeo et al., 2006)

Progenitor genotype

Carried in construct

P{UASp-Pp2B-14D.act}

Cytology

Description

UASp regulatory sequences drive expression of a constitutively active form of Pp2B-14D.

(Takeo et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Pp2B-14D

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle, with Scer\GAL4^{VP16.nanos.UTR}

(Takeo et al., 2006)

female sterile, with Scer\GAL4^{VP16.nanos.UTR}

(Takeo et al., 2006)

Phenotype Manifest In

egg & nuclear chromosome, with Scer\GAL4^{VP16.nanos.UTR}

(Takeo et al., 2006)

egg & spindle, with Scer\GAL4^{VP16.nanos.UTR}

(Takeo et al., 2006)

oocyte & nuclear chromatin, with Scer\GAL4^{VP16.nanos.UTR}

(Takeo et al., 2006)

oocyte & nucleus, with Scer\GAL4^{VP16.nanos.UTR}

(Takeo et al., 2006)

Detailed Description

Statement

Reference

Females that express Pp2B-14D^{act.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} have fully developed ovaries but are sterile or semi-sterile, depending on the transgenic insertion. 10% of eggs produced by these females are normal, while 14% appear to have neither chromosomes nor spindles. The remaining 76% show 3 major phenotypes: 1. dispersed chromatins

with no obvious spindle (33%), 2. apparently normal chromosomes with an abnormal

spindle (38%) and 3. a mass of chromatin with an apparently normal spindle (5%). The nuclei of mature oocytes the majority had abnormal nuclei containing dispersed chromatins and that the remaining were arrested at metaphase I or

anaphase I.

(Takeo et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pp2B-14D^act.UASp, Scer\GAL4^{VP16.nanos.UTR} has female sterile phenotype, suppressible by sra^UASp.cTa, Scer\GAL4^{VP16.nanos.UTR}

(Takeo et al., 2006)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The sterility of females that express Pp2B-14D^{act.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, is effectively suppressed by coexpression of sra^{Scer\UAS.P\T.cTa}.

(Takeo et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pp2B-14D^{act.Scer\UAS.P\T}

Pp2B-14D^act.UASp

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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