FlyBase Allele Report: Dmel\tsr[139.cBa]

General Information

Symbol

Dmel\tsr^139.cBa

Species

D. melanogaster

Name

FlyBase ID

FBal0196160

Feature type

allele

Associated gene

Dmel\tsr

Associated Insertion(s)

Carried in Construct

P{tsr.139}

Also Known As

pP[C4cat-tsr¹³⁹]

Key Links

Transgenic product class

missense_variant

(Blair et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Blair et al., 2006)

Mutagen

in vitro construct

(Blair et al., 2006)

Progenitor genotype

tsr^+t6.4

(Blair et al., 2006)

Carried in construct

P{tsr.139}

Cytology

Description

Amino acid replacement: E139A.

(Blair et al., 2006)

Amino acid replacement: E140A.

(Blair et al., 2006)

Amino acid replacement: K141A.

(Blair et al., 2006)

Amino acid replacement: L142L.

(Blair et al., 2006)

Amino acid replacement: R143A.

(Blair et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

tsr

Encoded product / tool

tsr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity, with tsr^Δ96, tsr^V27Q.cBa

(Blair et al., 2006)

lethal | larval stage | heat sensitive, with tsr^Δ96

(Blair et al., 2006)

lethal | pupal stage | cold sensitive, with tsr^Δ96

(Blair et al., 2006)

lethal - all die before end of larval stage | heat sensitive, with tsr^Δ96, tsr^V27Q.cBa

(Blair et al., 2006)

lethal - all die before end of pupal stage | heat sensitive, with tsr^V27Q.cBa

(Blair et al., 2006)

some die during larval stage | heat sensitive, with tsr^Δ96, tsr^V27Q.cBa

(Blair et al., 2006)

some die during pupal stage | heat sensitive, with tsr^V27Q.cBa

(Blair et al., 2006)

viable | cold sensitive, with tsr^V27Q.cBa

(Blair et al., 2006)

viable | cold sensitive, with tsr^Δ96, tsr^V27Q.cBa

(Blair et al., 2006)

Phenotype Manifest In

microchaeta, with tsr^Δ96, tsr^V27Q.cBa

(Blair et al., 2006)

wing hair, with tsr^Δ96, tsr^V27Q.cBa

(Blair et al., 2006)

Detailed Description

Statement

Reference

Animals carrying both tsr^V27Q.cBa and tsr^139.cBa transgenes survive and are relatively healthy at 16^oC and 18^oC, but few animals survive to the late pupal stage at 25^oC.

Over 90% of tsr^V27Q.cBa, tsr^139.cBa; tsr^Δ96 flies raised at 18^oC show planar cell polarity defects. For example, wing hairs have a non-distal orientation. The site of wing prehair initiation is variable in these mutants while initiation occurs at the distal side of cells in wild type. In moderately to severely affected tsr^V27Q.cBa, tsr^139.cBa; tsr^Δ96 wings, prehairs are observed as F-actin accumulations near cell centres or as long F-actin fibers that span from near cell centres to the distal side of cells. Unlike in wild type, prehairs are not oriented along the PD axis but emerge from either side adjacent to the distal-most vertex of the cell. Additionally, F-actin accumulation on apical cell surfaces is increased and variable compared to wild-type wing cells.

The fine hairs of the notum often adopt non-posterior orientations in tsr^V27Q.cBa, tsr^139.cBa; tsr^Δ96 flies. Occasionally, multiple notum hairs are observed.

(Blair et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

tsr^139.cBa partially rescues tsr^Δ96

(Blair et al., 2006)

Comments

Expression of tsr^139.cBa results in a limited, temperature-dependent rescue of tsr^Δ96 mutants. At 29^oC, lethality is not rescued at all, at 25^oC, a few tsr^139.cBa; tsr^Δ96 animals survive to the second instar larval stage, and at 18^oC and 16^oC these animals are pupal lethal. Coexpression of both tsr^139.cBa and tsr^V27Q.cBa results in a more effective rescue, although this is still temperature-dependent. While tsr^V27Q.cBa, tsr^139.cBa; tsr^Δ96 animals die at the first larval instar at 29^oC, a few survive to the pupal stage at 25^oC, while animals survive to the adult stage at 16^oC and 18^oC.

(Blair et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

tsr^139.cBa

tsr¹³⁹

(Blair et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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