FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\spokRNAi.UAS
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General Information
Symbol
Dmel\spokRNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0197747
Feature type
allele
Associated gene
Dmel\spok
Associated Insertion(s)
Carried in Construct
P{UAS-spok.RNAi}
Also Known As
UAS-spok-IR, spok RNAi
Key Links
Transgenic product class
RNAi_reagent
(Ono et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Ono et al., 2006)
Mutagen
in vitro construct
(Ono et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-spok.RNAi}
Cytology
Description

UASt regulatory sequences drive expression of an inverse repeat that corresponds to 450bp of spok sequence.

(Ono et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
spok
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of spokdsRNA.Scer\UAS under the control of Scer\GAL4phm.PO, resulting in spok dsRNA, results in first larval instar stage lethality. Feeding Scer\GAL4phm.PO>spokdsRNA.Scer\UAS larvae with 20E, E or 2dE allows a significant percentage to survive to progress through all three larval molts. Feeding these larvae with ketiodiol allows a significant fraction to survive past the first molt but not past the second. Feeding with C or 7dC has no rescuing effect.

      (Ono et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of spoScer\UAS.T:Ivir\HA1 suppresses the first instar larval arrest phenotype seen when spokdsRNA.Scer\UAS is expressed under the control of Scer\GAL4phm.PU.

      (Komura-Kawa et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Transcription from the P{UAS-spok.IR} construct should produce spok dsRNA, resulting in dsRNA interference (RNAi) of the spok gene.

      (Ono et al., 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      spokRNAi.UAS
      spokdsRNA.Scer\UAS
      spokdsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (6)