FlyBase Allele Report: Dmel\AP-2α[GD5059]

General Information

Symbol

Dmel\AP-2α^GD5059

Species

D. melanogaster

Name

FlyBase ID

FBal0198529

Feature type

allele

Associated gene

Dmel\AP-2α

Associated Insertion(s)

Carried in Construct

P{GD5059}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD5059}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD5059

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

abnormal neuroanatomy | pupal stage P5, with Scer\GAL4^ppk.PU

(Zong et al., 2018)

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

abnormal size | pupal stage P5, with Scer\GAL4^ppk.PU

(Zong et al., 2018)

lethal, with Scer\GAL4^elav.PLu

(Dietzl et al., 2007)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

dendritic tree | pupal stage P5, with Scer\GAL4^ppk.PU

(Zong et al., 2018)

larval dorsal multidendritic neuron ddaC | pupal stage P5, with Scer\GAL4^ppk.PU

(Zong et al., 2018)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

Detailed Description

Statement

Reference

Expression of AP-2α^GD5059 under the control of Scer\GAL4^ppk.PU (and Dicer-2, for efficient RNAi) results in mild but fully penetrant ddaC neuron dendrite pruning defects at 16h after puparium formation, as compared to controls.

(Zong et al., 2018)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an increase in the number of neuroblasts in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing AP-2α^GD5059 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

Adults expressing AP-2α^GD5059 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show no significant change in the kinetics of high temperature (46[o]C) induced paralysis compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in defects in locomotion or adult/pharate lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development