FlyBase Allele Report: Dmel\α-Spec[GD9695]

General Information

Symbol

Dmel\α-Spec^GD9695

Species

D. melanogaster

Name

FlyBase ID

FBal0198544

Feature type

allele

Associated gene

Dmel\α-Spec

Associated Insertion(s)

Carried in Construct

P{GD9695}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD9695}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD9695

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

photoreceptor cell R8, with Scer\GAL4^GMR.long

(Pojer et al., 2021)

Detailed Description

Statement

Reference

The expression of α-Spec^GD9695 under the control of Scer\GAL4^GMR.long leads to an increase in the ratio of pale vs yellow photoreceptor cells R8.

(Pojer et al., 2021)

Adults expressing α-Spec^GD9695 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in a weak flyer phenotype.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9695

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL80^ts.αTub84B, α-Spec^GD9695, Scer\GAL4^da.PU is a non-enhancer of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL80^ts.αTub84B, α-Spec^GD9695, Scer\GAL4^da.PU is a non-suppressor of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

α-Spec^GD9695, Scer\GAL4^GMR.long has photoreceptor cell R8 phenotype, suppressible by yki^KK109756, Scer\GAL4^GMR.long

(Pojer et al., 2021)

Other

Statement

Reference

α-Spec^GD9695, Scer\GAL4^GMR.long, yki^KK109756 has photoreceptor cell R8 phenotype

(Pojer et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of yki^KK109756 suppresses the increased ratio of pale vs yellow photoreceptor cells R8 induced by the expression of α-Spec^GD9695 under the control of Scer\GAL4^GMR.long, even leading to all photoreceptor cells R8 becoming the yellow type.

(Pojer et al., 2021)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v25387

w¹¹¹⁸; P{GD9695}v25387

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9695

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

α-Spec^GD9695

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

Open Close